PMID: 9003403Jan 1, 1997Paper

Regulation of cell surface GLUT4 in skeletal muscle of transgenic mice

The Biochemical Journal
J T BrozinickE M Gibbs

Abstract

Marked overexpression of the glucose transporter GLUT4 in skeletal muscle membrane fractions of GLUT4 transgenic (TG) mice is accompanied by disproportionately small increases in basal and insulin-stimulated glucose transport activity. Thus we have assessed cell surface GLUT4 by photolabelling with the membrane-impermeant reagent 2-N-[4-(1-azi-2,2,2-trifluoroethyl)benzoyl]-1, 3-bis(D-mannos-4-yloxy)-2-propylamine (ATB-BMPA) and measured the corresponding glucose transport activity using 2-deoxyglucose in isolated extensor digitorum longus (EDL) muscles from non-transgenic (NTG) and GLUT4 TG mice in the absence and presence of 13.3 nM (2000 mu units/ml) insulin, without or with hypoxia as a model of muscle contraction. TG mice displayed elevated rates of glucose transport activity under basal and insulin-stimulated conditions, and in the presence of insulin plus hypoxia, compared with NTG mice. Photoaffinity labelling of cell surface GLUT4 indicated corresponding elevations in plasma membrane GLUT4 in the basal and insulin-stimulated states, and with insulin plus hypoxia, but no difference in cell surface GLUT4 during hypoxia stimulation. Subcellular fractionation of hindlimb muscles confirmed the previously observed 3-fold over...Continue Reading

Citations

Sep 15, 2004·Biochimica Et Biophysica Acta·Rodney A HillArieh Gertler
Aug 1, 2002·Oncogene·Rebecca B Scarr, Phillip A Sharp
Oct 3, 2015·Journal of Business Ethics : JBE·Timothy C Johnson
Jul 14, 1998·Insect Molecular Biology·M AshburnerJ J Peloquin
Nov 4, 2006·Journal of Applied Physiology·Abram Katz
Mar 10, 2011·American Journal of Physiology. Endocrinology and Metabolism·Naveen SharmaGregory D Cartee
Feb 15, 2001·American Journal of Physiology. Endocrinology and Metabolism·D D BelkeD L Severson
May 15, 2002·American Journal of Physiology. Endocrinology and Metabolism·Tetsuya YamadaYoshitomo Oka
Jun 5, 1998·The American Journal of Physiology·T H ReynoldsS W Cushman
Jun 8, 2013·The Journal of Biological Chemistry·Michael G LöfflerJonathan S Bogan

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