Regulation of Cell Volume by Nanosecond Pulsed Electric Fields.

The Journal of Physical Chemistry. B
Qi YangTakakazu Nakabayashi


Stimulation of cells by nanosecond pulsed electric fields (nsPEFs) has attracted attention as a technology for medical applications such as cancer treatment. nsPEFs have been shown to affect intracellular environments without significant damage to cell membranes; however, the mechanism underlying the effect of nsPEFs on cells remains unclear. In this study, we constructed electrodes for applying nsPEFs and analyzed the change in volume of a single cell due to nsPEFs using fluorescence and Raman microscopy. It was shown that the direction of the change depended on the applied electric field; expansion due to the influx of water was observed at high electric field, and cell shrinkage was observed at low electric field. The change in cell volume was correlated to the change in the intracellular Ca2+ concentration, and nsPEFs-induced shrinking was not observed when the Ca2+-free medium was used. This result suggests that the cell shrinkage is related to the regulatory volume decrease where the cell adjusts the increase in intracellular Ca2+ concentration, inducing the efflux of ions and water from the cell.


May 1, 1989·Basic Research in Cardiology·P C Dartsch, E Betz
Nov 1, 1974·Biophysical Journal·U ZimmermannF Riemann
Nov 20, 2002·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Martin JakabMarkus Paulmichl
Dec 20, 2003·DNA and Cell Biology·Stephen J BeebeKarl H Schoenbach
Mar 18, 2004·The Journal of Biological Chemistry·Jody A WhiteStephen J Beebe
May 6, 2006·Journal of Biomedical Optics·Yinghua SunLaura Marcu
Jun 9, 2007·Archives of Biochemistry and Biophysics·Andrei G PakhomovKarl H Schoenbach
Apr 30, 2009·Annual Review of Biomedical Engineering·Deok-Ho KimYu Sun
Oct 6, 2009·Circulation Journal : Official Journal of the Japanese Circulation Society·Joji Ando, Kimiko Yamamoto
Feb 23, 2011·Tissue Engineering. Part B, Reviews·Josephine C BodleElizabeth G Loboa
Sep 22, 2011·Archives of Biochemistry and Biophysics·Keiko Morotomi-YanoKen-ichi Yano
Oct 8, 2011·British Journal of Cancer·M KeidarB Trink
Dec 21, 2011·Proceedings of the National Academy of Sciences of the United States of America·Masaya OkadaKatsumasa Fujita
Dec 12, 2012·Biochimica Et Biophysica Acta·Iurii SemenovAndrei G Pakhomov
Jul 31, 2013·PloS One·Olga N PakhomovaAndrei G Pakhomov
Jul 1, 2014·Biochimica Et Biophysica Acta·Olga N PakhomovaAndrei G Pakhomov
Jul 21, 2016·The Journal of Membrane Biology·Esin B SözerP Thomas Vernier
Dec 18, 2016·Cellular and Molecular Life Sciences : CMLS·Elena C GianulisAndrei G Pakhomov
Oct 13, 2017·The Journal of Physical Chemistry Letters·Mizuki TakeuchiTakakazu Nakabayashi
Nov 6, 2018·The Biochemical Journal·Jonathan Rojo-RuizJavier García-Sancho
Feb 13, 2020·Angewandte Chemie·Toshiki SugimuraTakakazu Nakabayashi
Sep 29, 2020·Physical Chemistry Chemical Physics : PCCP·Hiroaki TakahashiTakakazu Nakabayashi

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.