Regulation of cellulases and xylanases from a derepressed mutant of Cellulomonas flavigena growing on sugar-cane bagasse in continuous culture

Bioresource Technology
Teresa Ponce-Noyola, M de la Torre

Abstract

When the wild type Cellulomonas flavigena was grown on glycerol, xylose or cellobiose, it produced basal levels of carboxymethyl-cellulase (CMCase), filter-paperase (FPase) and xylanase activities. By comparison, a catabolic derepressed mutant strain of the same organism produced markedly higher levels of these enzymes when grown on the same carbon sources. Sugar-cane bagasse induced both the wild type and the mutant strain to produce three- to eight-time higher levels of FPase and xylanase than was observed with xylose or cellobiose. Continuous culture was used to determine the minimal cellobiose or glucose concentrations that repress the enzyme synthesis in both strains. 2.5 g l(-1) glucose repressed FPase and xylanases from wild type, while 1.6 times more glucose was needed to repress the same activities in the PN-120 strain. In the same way, twofold more cellobiose was needed to reduce by 75% the CMCase and xylanase activities in the mutant compared to the wild type. The FPase in the presence of 4 g l(-1) cellobiose did not change in the same strain. Therefore, its derepressed and feedback resistant characters of PN-120 mutant are evident. On the other hand, isoelectrofocused crude extracts of mutant and wild strains induce...Continue Reading

References

Jan 1, 1989·Critical Reviews in Biotechnology·V S Bisaria, S Mishra
Jan 1, 1981·Annual Review of Biochemistry·S C Hubbard, R J Ivatt
Jan 1, 1994·FEMS Microbiology Reviews·P Béguin, J P Aubert

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Citations

Aug 25, 2009·Archives of Microbiology·Jesús Antonio Herrera-HerreraTeresa Ponce-Noyola
Mar 6, 2007·Current Microbiology·Gaspar A Barrera-IslasTeresa Ponce-Noyola
Jun 1, 2011·World Journal of Microbiology & Biotechnology·Sushma Deepthi ArliK C Patel
Jun 1, 2007·Indian Journal of Microbiology·B K Mishra, A K Pandey Lata
Sep 28, 2007·Applied Microbiology and Biotechnology·Leticia M Sánchez-HerreraTeresa Ponce-Noyola
Aug 31, 2010·Journal of Industrial Microbiology & Biotechnology·Oscar A Rojas-RejónTeresa Ponce-Noyola
Oct 12, 2014·Bioresource Technology·Pratima Gupta, Piyush Parkhey
Feb 23, 2013·Bioresource Technology·Tian-Qing LanXiaoguang Liu
May 23, 2007·Journal of Dairy Science·J-S Eun, K A Beauchemin

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