Regulation of cGMP-dependent protein kinase expression by Rho and Kruppel-like transcription factor-4.

The Journal of Biological Chemistry
Ying ZengRenate B Pilz

Abstract

Type I cGMP-dependent protein kinase (PKG I) plays a major role in vascular homeostasis by mediating smooth muscle relaxation in response to nitric oxide, but little is known about the regulation of PKG I expression in smooth muscle cells. We found opposing effects of RhoA and Rac1 on cellular PKG I expression: (i) cell density-dependent changes in PKG I expression varied directly with Rac1 activity and inversely with RhoA activity; (ii) RhoA activation by calpeptin suppressed PKG I, whereas RhoA down-regulation by small interfering RNA increased PKG I expression; and (iii) PKG I promoter activity was suppressed in cells expressing active RhoA or Rho-kinase but was enhanced in cells expressing active Rac1 or a dominant negative RhoA. Sp1 consensus sequences in the PKG I promoter were required for Rho regulation and bound nuclear proteins in a cell density-dependent manner, including the Krüppel-like factor 4 (KLF4). KLF4 was identified as a major trans-acting factor at two proximal Sp1 sites; active RhoA suppressed KLF4 DNA binding and trans-activation potential on the PKG I promoter. Experiments with actin-binding agents suggested that RhoA could regulate KLF4 via its ability to induce actin polymerization. Regulation of PKG I...Continue Reading

References

Feb 1, 1987·Molecular and Cellular Biology·J R de WetS Subramani
Sep 29, 1995·The Journal of Biological Chemistry·S BagrodiaR A Cerione
Aug 1, 1997·Trends in Biochemical Sciences·S M LohmannH R De Jonge
Aug 10, 2000·The Journal of Cell Biology·N K NorenB Kreft
Aug 16, 2000·American Journal of Physiology. Cell Physiology·T J Koh, J G Tidball
Sep 12, 2000·Nature Cell Biology·P Z AnastasiadisA B Reynolds
Sep 22, 2000·Trends in Cell Biology·M Symons, J Settleman
Oct 14, 2000·Arteriosclerosis, Thrombosis, and Vascular Biology·P G AndersonT M Lincoln
Nov 1, 2000·Cell·D Bar-Sagi, A Hall
Dec 15, 2000·Current Biology : CB·S M SchoenwaelderK Burridge
Jan 4, 2001·The International Journal of Biochemistry & Cell Biology·D T DangV W Yang
Dec 6, 2001·Nature Reviews. Molecular Cell Biology·M Fukata, K Kaibuchi
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Apr 16, 2002·Molecular Biology of the Cell·Maria Grazia LampugnaniElisabetta Dejana
Jun 26, 2002·The Journal of Biological Chemistry·Darren E CasteelRenate B Pilz
Jun 28, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Georg NickenigMichael Böhm
Feb 28, 2003·The Journal of Biological Chemistry·Nicole K NorenKeith Burridge

❮ Previous
Next ❯

Citations

Sep 21, 2012·The Journal of Biological Chemistry·Darren E CasteelRenate B Pilz
Mar 14, 2009·The Journal of Biological Chemistry·Hema RangaswamiRenate B Pilz
Aug 31, 2010·Stem Cells and Development·Changqing XieY Eugene Chen
Nov 11, 2010·Journal of Hypertension·Takaki HataUNKNOWN ROCK Study Group
Aug 19, 2010·Pharmacological Reviews·Sharron H FrancisDavid Sibley
Nov 9, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Daming ZhuangAviv Hassid
Dec 22, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Patricia R BachillerJesse D Roberts
Dec 25, 2010·Molecular and Cellular Endocrinology·Carlos Pérez-MonterLeonor Pérez-Martínez
Dec 8, 2007·Journal of Cellular Physiology·Anna A BirukovaKonstantin G Birukov
Sep 26, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Jeremiah S HinsonChristopher P Mack
Jul 8, 2008·Acta Biochimica Et Biophysica Sinica·Paul M Evans, Chunming Liu

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.