Regulation of chemokine gene expression and secretion in Staphylococcus aureus-infected osteoblasts

Microbes and Infection
Kathleen M Wright, Jon S Friedland

Abstract

Staphylococcus aureus is the major cause of osteomyelitis or bone infection, leading to major morbidity, often in children. Little is known about immunopathogenesis of osteomyelitis, although uncontrolled inflammation is a major clinical feature. This study investigated effects of dexamethasone, PGE(2) and T(h)2 cytokines, all potential down-regulatory mediators, on control of S. aureus-induced C-X-C (CXCL8, CXCL10) and C-C (CCL5, CCL2) chemokine gene expression and secretion from human osteoblastic MG-63 cells and primary NHOst cells. Chemokine mRNA expression and secretion were reduced 50-75% by dexamethasone, whereas PGE(2) doubled mRNA accumulation, as detected by RNase protection assay and RT-PCR, but decreased chemokine secretion 33-71% (P < 0.05). IL-10 reduced chemokine mRNA accumulation by 20-40% in MG-63 cells. IL-4 and -13 decreased CXCL8 but not CXCL10 gene expression. IL-10 and IL-13 reduced S. aureus-induced osteoblast C-X-C chemokine secretion, whereas IL-4 decreased CXCL8 secretion 2.5-fold and increased CXCL10 secretion 3-fold (all P < 0.05). In contrast, T(h)2 cytokines increased C-C chemokine secretion from MG-63 osteoblastic cells (P < 0.05), and IL-4 and IL-13 caused similar up-regulation of CCL2 secretion ...Continue Reading

Citations

Oct 30, 2012·Scandinavian Journal of Infectious Diseases·Sifeng Shi, Xianlong Zhang
Dec 5, 2015·Frontiers in Cellular and Infection Microbiology·Jérôme JosseSophie C Gangloff
Jul 26, 2016·Frontiers in Cellular and Infection Microbiology·Jérôme JosseSophie C Gangloff
Mar 7, 2017·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Johannes M WagnerBjörn Behr
Mar 27, 2007·Journal of Dental Research·T A SilvaF Q Cunha
Dec 22, 2017·Photochemistry and Photobiology·Taisa Nogueira PansaniCarlos Alberto de Souza Costa
Sep 13, 2012·Experimental and Therapeutic Medicine·Sifeng Shi, Xianlong Zhang
Sep 1, 2012·Journal of Applied Physiology·Juliano M MartinsGlória E P Souza
Feb 24, 2010·Chemico-biological Interactions·Vicky P ChenKarl W K Tsim

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

CRISPR & Staphylococcus

CRISPR-Cas system enables the editing of genes to create or correct mutations. Staphylococci are associated with life-threatening infections in hospitals, as well as the community. Here is the latest research on how CRISPR-Cas system can be used for treatment of Staphylococcal infections.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.