Regulation of cholesterol synthesis in four colonic adenocarcinoma cell lines

Lipids
S R CerdaS A Broitman

Abstract

Colon tumor cells, unlike normal human fibroblasts, exhibited an uncoupling of low density lipoprotein (LDL)-derived cholesterol from cellular growth, when endogenous cholesterol synthesis was inhibited by mevinolin, a hydroxymethylglutaryl-CoA reductase (HMG-CoAR) competitive inhibitor [Fabricant, M., and Broitman, S.A. (1990) Cancer Res. 50, 632-636]. Further evaluation of cholesterol metabolism was conducted in two undifferentiated (SW480, SW1417) and two differentiated (HT29, CACO2) colonic adenocarcinoma (adeno-CA) cell lines and an untransformed human fibroblast, AG1519A. Cells grown in monolayer culture to near subconfluency were used to assess endogenous cholesterol synthesis by 14C-acetate incorporation, in response to the following treatments in lipoprotein-deficient serum (LPDS)-supplemented minimum essential medium (MEM): LPDS alone, LDL, mevinolin, mevinolin with LDL, and 25-hydroxy-cholesterol (25-OH-CH). Complete fetal bovine serum (FBS)-supplemented MEM was used as control. All colon tumor lines exhibited similarly high endogenous cholesterol synthesis in both FBS and LPDS relative to the fibroblasts which demonstrated low basal levels in FBS and maximal synthesis in LPDS. LDL treatment did not inhibit cholester...Continue Reading

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Citations

Nov 2, 2012·International Journal of Colorectal Disease·Thomas BroughtonIan L P Beales
Sep 25, 1999·Bioorganic & Medicinal Chemistry Letters·S K Panda, S A Broitman
Jan 30, 2013·Biochimica Et Biophysica Acta·James Robert Krycer, Andrew John Brown
May 4, 2010·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Luca PuglieseElisabetta Albi
Dec 30, 2015·Journal of the American Society of Nephrology : JASN·Karam AboudehenPeter Igarashi
Jun 22, 1999·British Journal of Cancer·S K PandaS A Broitman
Jan 22, 2019·Frontiers in Pharmacology·Huanbiao MoHoda Yeganehjoo
May 29, 2009·Journal of Medicinal Chemistry·João F S CarvalhoM Luisa Sá E Melo

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