Regulation of choline acetyltransferase/lacZ fusion gene expression in putative cholinergic neurons of Drosophila melanogaster

Journal of Neurobiology
Toshihiro KitamotoP M Salvaterra

Abstract

We have analyzed the distribution of putative cholinergic neurons in whole-mount preparations of adult Drosophila melanogaster. Putative cholinergic neurons were visualized by X-gal staining of P-element transformed flies carrying a fusion gene consisting of 5' flanking DNA from the choline acetyltransferase (ChAT) gene and lacZ reporter gene. We have previously demonstrated that cryostat sections of transgenic flies carrying 7.4 kb of ChAT 5' flanking DNA show reporter gene expression in a pattern essentially similar to the known distribution of ChAT protein. Whole-mount staining of these same flies by X-gal should thus represent the overall distribution of ChAT-positive neurons. Extensive staining was observed in the cephalic, thoracic, and stomodeal ganglia, primary sensory neurons in antenna, maxillary palps, labial palps, leg, wing, and male genitalia. Primary sensory neurons associated with photoreceptors and tactile receptors were not stained. We also examined the effects of partial deletions of the 7.4 kb fragment on reporter gene expression. Deletion of the 7.4 kb fragment to 1.2 kb resulted in a dramatic reduction of X-gal staining in the peripheral nervous system (PNS). This indicates that important regulatory elemen...Continue Reading

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Feb 1, 1993·Roux's Archives of Developmental Biology : the Official Organ of the EDBO·Toshihiro Kitamoto, Paul M Salvaterra

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Citations

Mar 17, 2004·Brain Research. Gene Expression Patterns·P M Salvaterra, T Kitamoto
Jun 13, 2008·Nature·Fumika N HamadaPaul A Garrity
Jan 26, 2010·The Journal of Biological Chemistry·Mei-Chin LeeYan Luo
Sep 13, 2003·BMC Neuroscience·Subhabrata SanyalMani Ramaswami
Jul 19, 2012·PLoS Genetics·Yoshinori AsoHiromu Tanimoto
Sep 20, 2002·Proceedings of the National Academy of Sciences of the United States of America·Toshihiro Kitamoto
Nov 5, 1999·The Journal of Cell Biology·K RayL S Goldstein
Aug 5, 2015·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·David OwaldScott Waddell
Feb 28, 1998·The Journal of Biological Chemistry·T KitamotoP M Salvaterra
Mar 29, 2002·The Journal of Comparative Neurology·Kouji YasuyamaFriedrich-Wilhelm Schürmann
May 20, 1999·Microscopy Research and Technique·K Yasuyama, P M Salvaterra
Dec 4, 2003·The Journal of Comparative Neurology·Irina Sinakevitch, Nicholas J Strausfeld
Aug 30, 2005·The Journal of Comparative Neurology·Elena Sivan-Loukianova, Daniel F Eberl

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