Regulation of chondroitin sulfate biosynthesis by specific sulfation: acceptor specificity of serum beta-GalNAc transferase revealed by structurally defined oligosaccharides

Glycobiology
H KitagawaK Sugahara

Abstract

The relationship between sulfation and polymerization in chondroitin sulfate (CS) biosynthesis has been poorly understood. In this study, we investigated the specificity of bovine serum UDP-GalNAc: CS beta-GalNAc transferase responsible for chain elongation using structurally defined acceptor substrates. They consisted of tetra- and hexasaccharide-serines that were chemically synthesized and various regular oligosaccharides with a GlcA residue at the nonreducing terminus, prepared from chondroitin and CS using testicular hyaluronidase. The enzyme preparation was obtained from fetal bovine serum by means of heparin-Sepharose affinity chromatography. The preparation did not contain the alpha-GalNAc transferase recently demonstrated in fetal bovine serum (Kitagawa et al., J. Biol. Chem., 270, 22190-22195, 1995), that utilizes common acceptor substrates. The beta-GalNAc transferase used as acceptors, two hexasaccharide-serines GlcA beta 1-3GalNAc beta 1-4GlcA beta 1-3Gal beta 1-3Gal beta 1-4Xyl beta 1-O-Ser and GlcA beta 1-3GalNAc(4-sulfate) beta 1-4GlcA beta 1-3Gal (4-sulfate) beta 1-3Gal beta 1-4Xyl beta 1-O-Ser, but neither the monosulfated hexasaccharide-serine GlcA beta 1-3GalNAc(4-sulfate) beta 1-4GlcA beta 1-3Gal beta 1-3Gal...Continue Reading

Citations

Aug 6, 2013·Analytical Chemistry·Vitor H Pomin
Oct 24, 2000·Current Opinion in Structural Biology·K Sugahara, H Kitagawa
Dec 9, 2009·Expert Reviews in Molecular Medicine·Fardad T AfshariJames W Fawcett
Jul 26, 2002·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Hiroshi Kitagawa
Aug 22, 2001·The Journal of Biological Chemistry·H KitagawaK Sugahara
Apr 2, 2003·The Journal of Biological Chemistry·Mats JönssonAke Oldberg
Sep 22, 2007·The Journal of Pharmacology and Experimental Therapeutics·Noelia CañasManuela G López

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