Regulation of ciaXRH operon expression and identification of the CiaR regulon in Streptococcus mutans.

Journal of Bacteriology
Chenggang WuFengxia Qi

Abstract

The ciaRH operon in Streptococcus mutans contains 3 contiguous genes, ciaXRH. Unlike the CiaRH system in other streptococci, only the ciaH-null mutant displays defective phenotypes, while the ciaR-null mutant behaves like the wild type. The objective of this study was to determine the mechanism of this unusual property. We demonstrate that the ciaH mutation caused a >20-fold increase in ciaR transcript synthesis. A ciaRH double deletion reversed the ciaH phenotype, suggesting that overexpressed ciaR might be responsible for the observed ciaH phenotypes. When ciaR was forced to be overexpressed by a transcriptional fusion to the ldh promoter in the wild-type background, the same ciaH phenotypes were restored, confirming the involvement of overexpressed ciaR in the ciaH phenotypes. The ciaH mutation and ciaR overexpression also caused transcriptional alterations in 100 genes, with 15 genes upregulated >5-fold. Bioinformatics analysis identified a putative CiaR regulon consisting of 8 genes/operons, including the ciaXRH operon itself, all of which were upregulated. In vitro footprinting on 4 of the 8 promoters revealed a protected region of 26 to 28 bp encompassing two direct repeats, NTTAAG-n5-WTTAAG, 10 bp upstream of the -10 re...Continue Reading

References

Aug 31, 2000·Annual Review of Biochemistry·A M StockP N Goudreau
Feb 27, 2001·Journal of Bacteriology·E A Groisman
Jan 5, 2002·Molecular Plant-microbe Interactions : MPMI·S Heeb, D Haas
Dec 18, 2002·Journal of Bacteriology·Thorsten MascherRegine Hakenbeck
Jun 10, 2004·Microbiology and Molecular Biology Reviews : MMBR·Sylvie ElsenCarl E Bauer
Jan 6, 2005·Microbiology·Justin MerrittWenyuan Shi
Feb 24, 2006·Infection and Immunity·Sang-Joon AhnRobert A Burne
May 15, 2007·Journal of Bacteriology·Dragana Ajdić, Vi T T Pham
Oct 30, 2007·Journal of Bacteriology·Indranil BiswasSaswati Biswas
Sep 17, 2008·Advances in Experimental Medicine and Biology·Jiang Wu, Carl E Bauer

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Citations

Aug 16, 2011·Applied and Environmental Microbiology·Jeffrey A BanasMin Zhu
Apr 4, 2014·Current Oral Health Reports·Justin MerrittJens Kreth
Jan 24, 2013·Peptides·Bruno Rocha da SilvaEdson Holanda Teixeira
Oct 15, 2019·Frontiers in Microbiology·Zhiyan HeWei Zhou
Mar 20, 2021·Nature Microbiology·Caroline A WerlangKatharina Ribbeck
Aug 3, 2021·Frontiers in Microbiology·Li-Yuan HeXiao-Yan Yang

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