PMID: 9178619Jul 1, 1997Paper

Regulation of Cl- transport by IBMX in renal A6 epithelium

Pflügers Archiv : European journal of physiology
Naomi Niisato, Yoshinori Marunaka

Abstract

We studied regulation of Cl- transport by cAMP and Ca2+ in renal epithelial A6 cells. Stimulation of A6 cells by 1 mM 3-isobutyl-1-methylxanthine (IBMX, an inhibitor of phosphodiesterase), which increased cytosolic cAMP, elicited biphasic increases in short-circuit current (Isc), i.e., a transient phase followed by a sustained one. Apical application of 5-nitro-2-(3-phenylpropylamino)-benzoate (NPPB, a Cl- channel blocker) markedly and dose-dependently inhibited the IBMX-induced Isc. Pretreatment with nifedipine (100 microM, a Ca2+ channel blocker) or 1,2-bis(o-aminophenoxy)-ethane-N,N,N',N'-tetraacetic acid tetra-(acetoxymethyl)-ester (BAPTA/AM, 10 microM, a Ca2+ chelator) partially but markedly inhibited the Isc. On the other hand, a cAMP-dependent protein kinase inhibitor, H89 (0.5 microM for 1 h), also reduced the IBMX-induced Isc to a level similar to that following nifedipine or BAPTA pretreatment. Nifedipine had no synergistic effects on the IBMX-induced Isc in cells treated with H89. Ionomycin (a Ca2+ ionophore) could mimic the transient increase dose dependently, and H89 did not block the ionomycin-induced Isc. Taken together, our observations suggest that: (1) part of the IBMX-stimulated Cl- release is regulated by an...Continue Reading

Citations

Jul 27, 2001·Biochemical and Biophysical Research Communications·N Niisato, Y Marunaka
Jun 1, 2005·Biochemical and Biophysical Research Communications·Tomoko Ueda-NishimuraYoshinori Marunaka
Nov 1, 2002·Biochemical and Biophysical Research Communications·Shinsaku TokudaYoshinori Marunaka
Sep 22, 2001·Toxicology in Vitro : an International Journal Published in Association with BIBRA·H F BjerregaardJ Vang
Nov 3, 2006·American Journal of Respiratory Cell and Molecular Biology·Makoto YasudaYoshinori Marunaka
Nov 18, 1998·Current Opinion in Nephrology and Hypertension·K Steinmeyer, T J Jentsch
Sep 4, 2007·Biochemical and Biophysical Research Communications·Makoto YasudaYoshinori Marunaka
Apr 3, 2007·Biochemical and Biophysical Research Communications·Naomi NiisatoYoshinori Marunaka
Jul 31, 2007·Biochemical and Biophysical Research Communications·Naomi NiisatoYoshinori Marunaka
Mar 21, 2006·Biochemical and Biophysical Research Communications·Isao HasegawaYoshinori Marunaka
Sep 1, 2005·Biochemical and Biophysical Research Communications·Shin-Ichiro FujimotoYoshinori Marunaka
Jul 21, 2001·Biophysical Journal·T G Păunescu, S I Helman
Mar 7, 2000·Toxicology and Applied Pharmacology·B Faurskov, H F Bjerregaard
Jul 6, 2000·The Japanese Journal of Physiology·M FukudaY Saek
Jan 29, 1999·Physiological Reviews·B D SchultzR J Bridges
Sep 14, 1999·The American Journal of Physiology·W ZeiskeW van Driessche

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