Oct 1, 1989

Regulation of collagen production by medial smooth muscle cells in hypoxic pulmonary hypertension

The American Review of Respiratory Disease
E C CrouchR P Mecham


Pulmonary hypertension is associated with abnormal connective tissue deposition in the media of pulmonary arteries. Lobar arteries from calves maintained for up to 15 days at simulated high altitude showed a 35% increase in collagen and a greater than 40% increase in crosslinked elastin per microgram protein. Labeling of artery tissue with [14C]proline revealed a nearly twofold increase in relative collagen synthesis. There was increased incorporation into Types I, III, IV, and V collagen with an increase in the proportion of newly synthesized Type IV collagen. Quantitation of collagen mRNA by slot-blot assay demonstrated increased levels of Types I and IV collagen message. In addition, medial smooth muscle cells isolated from the hypertensive calves demonstrated a nearly twofold increase in relative collagen synthesis, a twofold increase in the accumulation of newly synthesized collagen per microgram DNA, and increased levels of Types I and IV collagen mRNA. Exposure of pulmonary artery smooth muscle cells, adventitial cells, and fetal calf ligament fibroblasts to conditioned calf serum harvested from cultures of medial cells from hypertensive animals increased their levels of collagen as well as elastin mRNA. These studies su...Continue Reading

Mentioned in this Paper

Muscle, Smooth, Vascular
Structure of Calf of Leg
ELN gene
Arterial System
Specimen Type - Fibroblasts
Organ Culture Techniques
Cattle calf (organism)
Myocytes, Smooth Muscle
Bos taurus

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Bone Marrow Neoplasms

Bone Marrow Neoplasms are cancers that occur in the bone marrow. Discover the latest research on Bone Marrow Neoplasms here.

IGA Glomerulonephritis

IgA glomerulonephritis is a chronic form of glomerulonephritis characterized by deposits of predominantly Iimmunoglobin A in the mesangial area. Discover the latest research on IgA glomerulonephritis here.

Cryogenic Electron Microscopy

Cryogenic electron microscopy (Cryo-EM) allows the determination of biological macromolecules and their assemblies at a near-atomic resolution. Here is the latest research.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

LRRK2 & Immunity During Infection

Mutations in the LRRK2 gene are a risk-factor for developing Parkinson’s disease. However, LRRK2 has been shown to function as a central regulator of vesicular trafficking, infection, immunity, and inflammation. Here is the latest research on the role of this kinase on immunity during infection.

Antiphospholipid Syndrome

Antiphospholipid syndrome or antiphospholipid antibody syndrome (APS or APLS), is an autoimmune, hypercoagulable state caused by the presence of antibodies directed against phospholipids.

Meningococcal Myelitis

Meningococcal myelitis is characterized by inflammation and myelin damage to the meninges and spinal cord. Discover the latest research on meningococcal myelitis here.

Alzheimer's Disease: MS4A

Variants within membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease by recent genome-wide association studies. Here is the latest research.