Regulation of connexin43 oligomerization is saturable

Cell Communication & Adhesion
Jayasri Das SarmaMichael Koval

Abstract

We have used connexin constructs containing a C-terminal di-lysine-based endoplasmic reticulum (ER) retention/retrieval signal (HKKSL) transfected into HeLa cells to study early events in connexin oligomerization. Using this approach, we found that Cx43-HKKSL stably expressed at moderate levels by HeLa cells was retained in the ER and prevented from oligomerization. However, Cx43-HKKSL stably overexpressed by HeLa cells escaped from the ER and localized to a perinuclear region of the cell that included the Golgi apparatus. Overexpressed Cx43-HKKSL oligomerized into hexamers and also formed Triton X-100 insoluble, intracellular complexes that resembled gap junctions. Thus, the ability of HeLa cells to inhibit Cx43 oligomerization was saturable. HeLa cells stably overexpressing Cx43-HKKSL may provide a useful model system to evaluate pharmacologic agents and/or cDNAs encoding chaperones with the potential to regulate initial steps in Cx43 oligomerization.

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Citations

Jun 23, 2012·The Journal of Membrane Biology·Tekla D SmithMichael Koval
Sep 15, 2009·Oncogene·C P K LaiC C Naus
Feb 1, 2011·Antioxidants & Redox Signaling·Christian E OvergaardMichael Koval
Mar 27, 2009·Molecular Biology of the Cell·Shamie DasMichael Koval
May 4, 2012·Critical Reviews in Biochemistry and Molecular Biology·Jérôme GilleronGeorges Pointis
Jul 24, 2008·Cell Communication & Adhesion·Jayasri Das SarmaMichael Koval
Feb 24, 2006·Trends in Cell Biology·Michael Koval
Nov 16, 2014·Cell and Tissue Research·John J KellyDale W Laird
Oct 6, 2020·Frontiers in Physiology·Margaret BreckerRonald C Rubenstein
Aug 19, 2007·The Journal of Biological Chemistry·Brandy L DaughertyMichael Koval
Aug 13, 2015·The Journal of Biological Chemistry·Randy F StoutDavid C Spray
Dec 2, 2020·Biomolecules·Joell L Solan, Paul D Lampe

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