PMID: 9166723Jun 1, 1997Paper

Regulation of corticotropin-releasing factor receptor function in human Y-79 retinoblastoma cells: rapid and reversible homologous desensitization but prolonged recovery

Journal of Neurochemistry
R L HaugerJ Spiess

Abstract

Homologous receptor desensitization is an important regulatory response to continuous activation by agonist that involves the uncoupling of a receptor from its G protein. When human retinoblastoma Y-79 cells expressing corticotropin-releasing factor (CRF) receptors were preincubated with CRF for 10 min-4 h, a time-dependent reduction in both the peak and sensitivity of CRF-stimulated intracellular cyclic AMP (cAMP) accumulation developed with a t1/2 of 38 min and an EC50 of 6-7 nM CRF. CRF receptor desensitization was slowly reversible after a 4-h CRF preincubation with a t1/2 of 13 h and a full restoration of cAMP responsiveness to CRF at 24 h following the removal of 10 nM CRF. Because the ability of vasoactive intestinal peptide, forskolin, or (-)-isoproterenol to stimulate cAMP accumulation was not diminished in Y-79 cells desensitized with 10 nM CRF, the observed desensitization was considered to be a specific homologous action of CRF. CRF receptor desensitization was markedly attenuated by CRF receptor antagonists, which alone did not produce any appreciable reduction in CRF-stimulated cAMP accumulation. Although recent reports have demonstrated a rapid decline in steady-state levels of CRF receptor type 1 (CRF-R1) mRNA i...Continue Reading

Citations

Mar 15, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·F M DautzenbergR L Hauger
Mar 17, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Robert H OakleyRichard L Hauger
Nov 13, 2009·Annals of the New York Academy of Sciences·Richard L HaugerFrank M Dautzenberg
Aug 16, 2018·Physiological Reviews·Jan M Deussing, Alon Chen
Feb 10, 2010·Cellular and Molecular Life Sciences : CMLS·Athina KapraraEkaterini Chatzaki
Apr 6, 2002·Archives of Physiology and Biochemistry·D MasonP Thompson
Feb 19, 2000·Biochemical and Biophysical Research Communications·R L HaugerK J Catt
Apr 15, 2008·Trends in Endocrinology and Metabolism : TEM·J SpiessJ Radulovic
Mar 12, 1998·Brain Research. Molecular Brain Research·S SydowJ Spiess
May 5, 2001·Peptides·G AguileraM Nikodemova
May 12, 2004·Peptides·Greti AguileraKevin J Catt
Jul 3, 2010·European Journal of Pharmacology·Eric GutknechtFrank M Dautzenberg

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