PMID: 8979307Dec 1, 1996Paper

Regulation of COX-2 gene expression in rat uterus in vivo and in vitro

Prostaglandins
A Arslan, H H Zingg

Abstract

Prostaglandins are involved in mediating several important processes in mammalian reproduction, including the initiation of parturition. In the present study, we examined the expression in the rat uterus of two-rate limiting enzymes involved in prostaglandin production, cyclooxygenase (COX) 1 and 2. Expression of the COX-2 gene in the pregnant rat uterus gave rise to a single mRNA transcript of approximately 4.4 kb. COX-2 mRNA levels increased 3.5 fold between day 7 of pregnancy and the onset of parturition on day 22. In contrast, COX-1 mRNA levels remained constant during the same period. To investigate factors involved in mediating the regulation of COX-1 and COX-2 gene expression, rat endometrial stromal and epithelial cell lines, were used. In the stroma-derived cell line, CUS-V2, COX-2 gene expression was demonstrated by reverse transcriptase/polymerase chain reaction (RT-PCR) and by immunocytochemistry. In these cells, COX-2 gene expression was inducible by the cytokines interleukin-1 beta and tumor necrosis factor alpha, but not by interleukin-6. The two former cytokines also induced prostaglandin F2 alpha production. In contrast, COX-1 gene expression was constitutive in this cell line. In the endometrial epithelium-der...Continue Reading

References

Oct 1, 1992·American Journal of Obstetrics and Gynecology·R Romero, B Tartakovsky
Oct 1, 1992·American Journal of Obstetrics and Gynecology·R RomeroM D Mitchell
Jan 3, 1991·European Journal of Pharmacology·M D MitchellS L Schiller
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·L OsbornG J Nabel
Feb 28, 1989·Biochemical and Biophysical Research Communications·S A Jones, J R Challis
Jun 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·H Aviv, P Leder
Mar 15, 1969·Nature·F R Blatchley, B T Donovan
Jul 1, 1984·Endocrinology·H H ZinggJ F Habener
Nov 15, 1981·American Journal of Obstetrics and Gynecology·P HussleinF Fuchs
Feb 1, 1995·In Vitro Cellular & Developmental Biology. Animal·A ArslanH H Zingg
Nov 8, 1994·Proceedings of the National Academy of Sciences of the United States of America·R A CopelandJ M Trzaskos
Nov 15, 1994·Archives of Biochemistry and Biophysics·M D PercivalG P O'Neill
Dec 1, 1993·Archives of Biochemistry and Biophysics·L FengD Hwang
Sep 13, 1993·FEBS Letters·G P O'Neill, A W Ford-Hutchinson
Feb 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R J Mayer, L A Marshall

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Citations

Jan 1, 1997·Developmental Genetics·C L Stewart, E B Cullinan
Mar 12, 2003·Prostaglandins, Leukotrienes, and Essential Fatty Acids·J-C SchellenbergC C Van Ee
May 10, 2006·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·T-H ChenS Fujii
Feb 7, 2007·Acta Physiologica·N KarasawaH Tsuchida
Jul 29, 1998·The Journal of Pharmacy and Pharmacology·M H Yousif, O Thulesius
Mar 11, 2009·Journal of Biomedical Science·Huey-Chuan ShihLing-Ling Yang
Dec 21, 2006·The Journal of Reproduction and Development·Shiro KurusuMitsumori Kawaminami
Sep 8, 2000·Journal of the American Veterinary Medical Association·C J Jones, S C Budsberg
Jan 1, 2010·Polish Journal of Veterinary Sciences·M J SiemieniuchK Okuda
Mar 13, 2014·TheScientificWorldJournal·Naguib Salleh
Nov 6, 1998·Pediatric Research·R K Riemer, M A Heymann
May 8, 2007·Best Practice & Research. Clinical Obstetrics & Gynaecology·Katie M Groom
Oct 30, 2007·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Toru GamoMasa-Aki Hattori
Apr 6, 2004·Drug Safety : an International Journal of Medical Toxicology and Drug Experience·Dinesh KhannaDaniel E Furst
Feb 11, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Takashi KuwanoMayumi Ono
May 3, 2001·Obstetrical & Gynecological Survey·J A McGregorJ Morrison
May 15, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·T LahiriS A Shore
Feb 5, 2021·Environmental Pollution·Zijiang YangShiwen Xu
Aug 31, 2006·Prostaglandins & Other Lipid Mediators·S Pérez MartínezA Franchi

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