Regulation of Cripto-1 signaling and biological activity by caveolin-1 in mammary epithelial cells.

The American Journal of Pathology
Caterina BiancoDavid S Salomon

Abstract

Human and mouse Cripto-1 (CR-1/Cr-1) proteins play an important role in mammary gland development and tumorigenesis. In this study, we examined the relationship between Cripto-1 and caveolin-1 (Cav-1), a membrane protein that acts as a tumor suppressor in the mammary gland. Cripto-1 was found to interact with Cav-1 in COS7 cells and mammary epithelial cells. Using EpH4 mouse mammary epithelial cells expressing Cr-1 (EpH4 Cr-1) or Cr-1 and Cav-1 (EpH4 Cr-1/Cav-1), we demonstrate that Cav-1 expression markedly reduced the ability of Cr-1 to enhance migration, invasion, and formation of branching structures in EpH4 Cr-1/Cav-1 cells as compared to EpH4 Cr-1 cells. Furthermore, coexpression of Cav-1 together with Cr-1 in EpH4 Cr-1/Cav-1 cells inhibited Cr-1-mediated activation of c-src and mitogen-activated protein kinase signaling pathways. Conversely, primary mammary epithelial cells isolated from Cav-1 null(-/-)/mouse mammary tumor virus-CR-1 transgenic animals showed enhanced motility and activation of mitogen-activated protein kinase and c-src as compared to Cav-1(+/-)/CR-1 mammary cells. Finally, mammary tumors derived from mouse mammary tumor virus-CR-1 mice showed a dramatic reduction of Cav-1 expression as compared to mamma...Continue Reading

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Citations

Apr 28, 2011·Cellular and Molecular Neurobiology·Matthias SchmitzHans H Althaus
Aug 22, 2013·Micron : the International Research and Review Journal for Microscopy·Josiane AïounChristine Péchoux
Jul 14, 2010·Future Oncology·Nadia Pereira de CastroCaterina Bianco
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Dec 14, 2011·Growth Factors·Tadahiro NagaokaCaterina Bianco
Aug 26, 2014·Seminars in Cancer Biology·Malgorzata KlauzinskaDavid Salomon
Sep 13, 2018·Current Medicinal Chemistry·Annamaria Sandomenico, Menotti Ruvo
Sep 12, 2020·Oral Diseases·Hussein DaraghmaLuigi Strizzi
Nov 16, 2010·Expert Opinion on Therapeutic Patents·Caterina Bianco, David S Salomon
Jul 17, 2019·Journal of Cellular Biochemistry·Johann Mar Gudbergsson, Meg Duroux

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