Regulation of cyclin T1 during HIV replication and latency establishment in human memory CD4 T cells

Virology Journal
Jacob CouturierDorothy E Lewis

Abstract

The regulatory cyclin, Cyclin T1 (CycT1), is a host factor essential for HIV-1 replication in CD4 T cells and macrophages. The importance of CycT1 and the Positive Transcription Elongation Factor b (P-TEFb) complex for HIV replication is well-established, but regulation of CycT1 expression and protein levels during HIV replication and latency establishment in CD4 T cells is less characterized. To better define the regulation of CycT1 levels during HIV replication in CD4 T cells, multiparameter flow cytometry was utilized to study the interaction between HIV replication (intracellular p24) and CycT1 of human peripheral blood memory CD4 T cells infected with HIV in vitro. CycT1 was further examined in CD4 T cells of human lymph nodes. In activated (CD3+CD28 costimulation) uninfected blood memory CD4 T cells, CycT1 was most significantly upregulated in maximally activated (CD69+CD25+ and HLA.DR+CD38+) cells. In memory CD4 T cells infected with HIV in vitro, two distinct infected populations of p24+CycT1+ and p24+CycT1- cells were observed during 7 days infection, suggestive of different phases of productive HIV replication and subsequent latency establishment. Intriguingly, p24+CycT1- cells were the predominant infected population...Continue Reading

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Citations

Jul 21, 2020·AIDS Research and Human Retroviruses·Daniele C Cary, B Matija Peterlin
Dec 20, 2020·EBioMedicine·Lilly M Wong, Guochun Jiang
Apr 14, 2021·Current Hypertension Reports·Tashlen AbelThajasvarie Naicker
May 1, 2021·International Journal of Molecular Sciences·Tae-Hyeong Kim, Seong-Wook Lee

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Methods Mentioned

BETA
density-gradient centrifugation
PMA
flow cytometry
dotplots

Software Mentioned

PrimeFlow
Kaluza

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