Regulation of cyclooxygenase-2 expression by heat: a novel aspect of heat shock factor 1 function in human cells.

PloS One
Antonio RossiM Gabriella Santoro

Abstract

The heat-shock response, a fundamental defense mechanism against proteotoxic stress, is regulated by a family of heat-shock transcription factors (HSF). In humans HSF1 is considered the central regulator of heat-induced transcriptional responses. The main targets for HSF1 are specific promoter elements (HSE) located upstream of heat-shock genes encoding cytoprotective heat-shock proteins (HSP) with chaperone function. In addition to its cytoprotective function, HSF1 was recently hypothesized to play a more complex role, regulating the expression of non-HSP genes; however, the non-canonical role of HSF1 is still poorly understood. Herein we report that heat-stress promotes the expression of cyclooxygenase-2 (COX-2), a key regulator of inflammation controlling prostanoid and thromboxane synthesis, resulting in the production of high levels of prostaglandin-E(2) in human cells. We show that heat-induced COX-2 expression is regulated at the transcriptional level via HSF1-mediated signaling and identify, by in-vitro reporter gene activity assay and deletion-mutant constructs analysis, the COX-2 heat-responsive promoter region and a new distal cis-acting HSE located at position -2495 from the transcription start site. As shown by ChI...Continue Reading

References

Jan 21, 1997·Proceedings of the National Academy of Sciences of the United States of America·A RossiM G Santoro
Feb 21, 1998·Nucleic Acids Research·T HeinemeyerN A Kolchanov
Sep 10, 1999·Annals of Medicine·M Jäättelä
Sep 28, 2000·Current Opinion in Chemical Biology·L J Marnett
Feb 24, 2001·Cell Stress & Chaperones·J D Hasday, I S Singh
Jun 13, 2001·American Journal of Physiology. Cell Physiology·H ParfenovaC W Leffler
Nov 16, 2002·Prostaglandins & Other Lipid Mediators·Tadashi Tanabe, Norimitsu Tohnai
Dec 7, 2002·Trends in Biochemical Sciences·Carina I HolmbergLea Sistonen
Jan 9, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Linhua PangAlan J Knox
Jan 10, 2003·The Journal of Biological Chemistry·Lisa Q GothardKevin D Sarge
Jun 12, 2003·Cardiovascular Research·Claire ArnaudChristophe Ribuot
Dec 12, 2003·Molecular Biology of the Cell·Nathan D TrinkleinRichard M Myers
Dec 20, 2003·Nature·Christopher M Dobson
Jan 18, 2005·Physiology·Satoshi Murasawa, Takayuki Asahara
Jun 22, 2005·Prostaglandins & Other Lipid Mediators·Clark M BlatteisVit Perlik
Jun 13, 2006·Clinical Science·John D Imig
Sep 27, 2006·Handbook of Experimental Pharmacology·K Egan, G A FitzGerald
Dec 16, 2006·Endothelium : Journal of Endothelial Cell Research·Michel R HoenigJulie H Campbell
Jan 9, 2007·Advances in Experimental Medicine and Biology·Julius Anckar, Lea Sistonen
Feb 24, 2007·Progress in Lipid Research·Yeon-Joo KangWilliam L Smith
Dec 7, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Patience MurapaJerold G Woodward
Mar 28, 2008·American Journal of Respiratory Cell and Molecular Biology·Ishwar S SinghJeffrey D Hasday

❮ Previous
Next ❯

Citations

Jun 1, 2014·Current Opinion in Clinical Nutrition and Metabolic Care·Philip Newsholme, Paulo I Homem de Bittencourt
Jul 16, 2014·Cell Stress & Chaperones·Tiffany M BridgesJeffrey D Hasday
Jul 19, 2013·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·Ishwar S Singh, Jeffrey D Hasday
Jun 12, 2014·Kidney International·Shipra AgrawalWilliam E Smoyer
May 16, 2015·Nature Reviews. Immunology·Sharon S EvansDaniel T Fisher
Jan 28, 2014·PloS One·Srijit Das, Nitai Pada Bhattacharyya
Dec 1, 2017·American Journal of Physiology. Renal Physiology·Xiaojing NieWilliam E Smoyer
May 19, 2018·Cellular and Molecular Life Sciences : CMLS·János BarnaTibor Vellai
May 2, 2018·The Journal of Reproduction and Development·Shunsuke SakaiKiyoshi Okuda
Oct 12, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Asif AliMahadeb Pal
Mar 27, 2015·American Journal of Physiology. Renal Physiology·Inge CarlsenRikke Nørregaard
Jul 2, 2020·Cellular and Molecular Life Sciences : CMLS·Silvia SantopoloM Gabriella Santoro
Apr 20, 2021·Heliyon·Juan Sebastian Naranjo-GómezIang Schroniltgen Rondón-Barragán

❮ Previous
Next ❯

Methods Mentioned

BETA
acetylation
PCR
ELISA
confocal microscopy
ChIP
immunoprecipitation
FCS
transfection
Assay
electrophoresis

Software Mentioned

ECR Evolutionary Conserved Regions ( ECR ) browser
ImageQuant
TFSEARCH

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.