Regulation of cyclooxygenases by protein kinase C. Evidence against the importance of direct enzyme phosphorylation.

The Journal of Biological Chemistry
R VezzaG A FitzGerald

Abstract

Cyclooxygenases (COXs) are key prostaglandin biosynthetic enzymes. While COX-1 expression is largely constitutive, COX-2 is highly regulated by cytokines, growth factors, and tumor promoters, such as the protein kinase C (PKC) activator, phorbol 12-myristate 13-acetate (PMA). While phosphorylation of transcription factors may regulate COX transcription, the existence of PKC consensus sequences suggests that direct enzyme phosphorylation might also regulate differential expression of the enzymes. Nevertheless, phosphorylation of both human recombinant COX-1 and COX-2 by rat brain PKC in vitro was minimal, as was phosphorylation of peptides based on PKC consensus sequences in COX-1 (less than 4% of the phosphorylation of the PKC-alpha pseudosubstrate peptide). Similarly, phosphorylation of the corresponding COX-2 peptides was not observed using either the phosphocellulose paper absorption method or electrospray mass spectrometry. MEG-01 and NIH 3T3 cells were labeled with [32P]orthophosphate to investigate COX phosphorylation in vivo. COX-2 synthesis was induced by PMA (100 nM) or serum stimulation in NIH 3T3 cells. COX-1 was expressed constitutively in MEG-01 cells. Specific polyclonal antibodies raised against sequences of huma...Continue Reading

References

Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·T Hla, K Neilson
Aug 7, 1992·Cell·T Hunter, M Karin
Apr 28, 1995·The Journal of Biological Chemistry·D PraticoG A FitzGerald
May 26, 1995·The Journal of Biological Chemistry·G IwamiY Ishikawa
Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·O Jacobowitz, R Iyengar
Dec 6, 1994·Proceedings of the National Academy of Sciences of the United States of America·K SeibertP Isakson
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·J L MasferrerK Seibert
May 1, 1994·European Journal of Biochemistry·T KosakaT Tanabe
Jan 29, 1993·Cell·L L LinR J Davis
Aug 13, 1993·Cell·R J Lefkowitz
Jan 5, 1996·Biochimica Et Biophysica Acta·H R Herschman

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Citations

Aug 25, 2001·Growth Factors·C Mouta CarreiraT Maciag
Oct 9, 1998·The Journal of Investigative Dermatology·T KanekuraL R Ballou
Nov 5, 2003·Journal of Cellular and Molecular Medicine·O C Trifan, T Hla
Nov 25, 2004·Prostaglandins & Other Lipid Mediators·A GuzelogluW W Thatcher
Apr 18, 2001·Chemico-biological Interactions·M SotiropoulouM Marselos
Jan 24, 2008·Reproductive Sciences·Valentina CascianiJohn R G Challis
Nov 10, 2017·Journal of Hypertension·Brandi M WynneRobert S Hoover
Sep 25, 2017·Physiological Genomics·Anna Alexanian, Andrey Sorokin
May 20, 1999·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·M E SalesE S Borda
Apr 23, 1999·The Journal of Biological Chemistry·R VezzaG A FitzGerald
Oct 22, 2002·American Journal of Physiology. Cell Physiology·Lyne I LemieuxChris R J Kennedy
Feb 12, 1998·The American Journal of Physiology·H ParfenovaC W Leffler
May 17, 2001·Molecular Pharmacology·R VezzaG A FitzGerald
Jun 28, 2014·Oncotarget·Anna AlexanianAndrey Sorokin
Jul 20, 2021·Journal of Cellular Physiology·Santosh K YadavDeepak A Deshpande

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