PMID: 2870681Apr 1, 1986Paper

Regulation of cytochrome P-450c by glucocorticoids and polycyclic aromatic hydrocarbons in cultured fetal rat hepatocytes

Archives of Biochemistry and Biophysics
J M MathisE R Simpson


The actions of polycyclic aromatic hydrocarbons and glucocorticoids to regulate the synthesis of cytochrome P-450c (the major isozyme induced by polycyclic aromatic hydrocarbons) were investigated in fetal rat hepatocytes maintained in primary monolayer culture. Treatment of hepatocytes in culture with 1,2-benzanthracene resulted in a 50-fold increase in 7-ethoxycoumarin O-deethylase activity. The level of P-450c increased in the cells in a time-dependent fashion as determined by immunoelectrophoretic analysis. The inductive effect of BA was potentiated approximately 1.6- to 2.3-fold when 1 microM dexamethasone was included in the culture medium. However, dexamethasone alone had little or no effect on the induction of P-450c. The rate of synthesis of P-450c was examined by immunoisolation of the specific isozyme from total cellular proteins radiolabeled with [35S]methionine and from the protein products formed during in vitro translation of the isolated mRNA. In addition, the amount of mRNA specific for cytochrome P-450c was determined by Northern blot analysis of RNA extracted from cultured cells. The changes in the rates of synthesis and mRNA levels were found to parallel the changes in enzyme activity. The concentration of d...Continue Reading


Aug 28, 1979·Biochemical and Biophysical Research Communications·P CommerJ F Chiu
May 1, 1975·Journal of Steroid Biochemistry·O Greengard
Apr 1, 1976·Analytical Biochemistry·J R FryJ W Bridges
Mar 1, 1985·Archives of Biochemistry and Biophysics·R N HinesE Bresnick
May 15, 1985·Archives of Biochemistry and Biophysics·R L FoldesE Bresnick
Jul 15, 1982·Biochemical Pharmacology·D W Nebert, M Negishi
Jul 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J L VanniceG M Ringold
Sep 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·W H LamersH M Meisner
Dec 1, 1983·Archives of Biochemistry and Biophysics·J PapaconstantinouE Y Wong
Sep 1, 1983·Archives of Biochemistry and Biophysics·B TierneyE Bresnick
Jun 15, 1982·Biochemical and Biophysical Research Communications·T CresteilJ P Leroux
Jan 1, 1981·Biology of the Neonate·A HussonR Vaillant
Dec 28, 1971·Biochimica Et Biophysica Acta·J I Thorell, B G Johansson


Jan 1, 1990·Pharmacology & Therapeutics·A B Okey
Dec 1, 1988·Molecular and Cellular Endocrinology·J M MathisR A Prough
Mar 1, 1993·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·P C LeeJ J Lech
Jun 1, 1993·Journal of Pharmacological and Toxicological Methods·J L Raucy, S J Carpenter
Sep 6, 2001·Respiration Physiology·Y TesfaigziW Kozak
Apr 6, 2000·The Biochemical Journal·P Honkakoski, M Negishi
Aug 1, 1993·In Vitro Cellular & Developmental Biology. Animal·A K Dasmahapatra, P C Lee
May 21, 2010·Drug Metabolism Reviews·Zdenek Dvorak, Peotr Pavek
Jul 25, 2009·Drug Metabolism Reviews·Katalin MonostoryZdenek Dvorak
Aug 1, 1988·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·M Warren, J R Fry
Nov 26, 1986·Biochemical and Biophysical Research Communications·K T ShiverickM O James

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Spatio-Temporal Regulation of DNA Repair

DNA repair is a complex process regulated by several different classes of enzymes, including ligases, endonucleases, and polymerases. This feed focuses on the spatial and temporal regulation that accompanies DNA damage signaling and repair enzymes and processes.

Glut1 Deficiency

Glut1 deficiency, an autosomal dominant, genetic metabolic disorder associated with a deficiency of GLUT1, the protein that transports glucose across the blood brain barrier, is characterized by mental and motor developmental delays and infantile seizures. Follow the latest research on Glut1 deficiency with this feed.

Separation Anxiety

Separation anxiety is a type of anxiety disorder that involves excessive distress and anxiety with separation. This may include separation from places or people to which they have a strong emotional connection with. It often affects children more than adults. Here is the latest research on separation anxiety.

KIF1A Associated Neurological Disorder

KIF1A associated neurological disorder (KAND) is a rare neurodegenerative condition caused by mutations in the KIF1A gene. KAND may present with a wide range and severity of symptoms including stiff or weak leg muscles, low muscle tone, a lack of muscle coordination and balance, and intellectual disability. Find the latest research on KAND here.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.