Regulation of cytokine gene expression in experimental autoimmune encephalomyelitis

Journal of Neuroscience Research
I M ZitronR H Swanborg

Abstract

We previously reported that recovery of Lewis rats from experimental autoimmune encephalomyelitis (EAE) is associated with the appearance of suppressor T cells (Ts). These Ts secrete TGF-beta which down-regulates the production of inflammatory cytokines by the effector T cells that mediate this disease. In the present study, we immunized Lewis rats with myelin basic protein (MBP)+CFA, and evaluated purified T cells and MBP-activated spleen cells (SpC) during the paralytic phase (day 12) and after recovery (days 30-33) for TGF-beta and interferon (IFN)-gamma mRNA. We used reverse transcriptase-polymerase chain reaction (RT-PCR), quantitated on the basis of beta-actin mRNA. Abundant IFN-gamma mRNA was present in MBP-activated SpC obtained on day 12. In contrast, only trace IFN-gamma mRNA was detected in day 30 activated SpC, and no IFN-gamma mRNA was present in purified, nonactivated T cells obtained at either time. The level of IFN-gamma mRNA correlated with secretion of IFN-gamma as determined by ELISA on SpC culture supernatants, and with severity of adoptively transferred EAE by the activated SpC. Thus, it appears that IFN-gamma mRNA is both transcribed and translated in response to antigen activation, resulting in secretion ...Continue Reading

References

Apr 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·A P KuruvillaG J Thorbecke
May 25, 1990·Nucleic Acids Research·S W QianM B Sporn
Jan 1, 1988·Methods in Enzymology·R H Swanborg
Aug 1, 1994·Molecular and Chemical Neuropathology·P Dore-DuffyR H Swanborg

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Citations

May 16, 2000·European Journal of Neurology : the Official Journal of the European Federation of Neurological Societies·E WallströmT Olsson
Feb 20, 1998·Journal of Neuroscience Research·R B Smeltz, R H Swanborg

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