PMID: 7535753Mar 29, 1995Paper

Regulation of cytoskeletal organization in tumor cells by protein phosphatases-1 and -2A

International Journal of Cancer. Journal International Du Cancer
G D MaierM R Young

Abstract

Non-metastatic Lewis lung carcinoma cells (LLC-C8) become more motile when protein phosphatases (PP-1 and -2A) are inhibited by okadaic acid, attaining the same level of motility as metastatic LLC (LLC-LN7) variants. This stimulation of LLC-C8 motility was tempered when protein kinase A activity was inhibited. We examined whether the okadaic acid-stimulated LLC-C8 motility was associated with alterations in the cytoskeletal organization so that these non-metastatic cells acquire the rounded morphology and diffuse cytoskeletal organization previously described for metastatic LLC-LN7 cells. Non-metastatic LLC-C8 are typically adherent during culture, achieving a spread morphology. Treatment of non-metastatic LLC-C8 cells with okadaic acid resulted in a contraction of most of their extended processes, formation of spikes and membrane blebs within 10 min, and complete cell rounding within 20 min for most of the cells. While the overall level of F-actin was minimally affected by the okadaic acid, its uniform distribution shifted to localization toward the periphery of the rounded cells, often concentrating at a single focus. Immunofluorescent staining for vimentin showed a similar shift to the cell periphery and similar capping. Aft...Continue Reading

References

Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J E ErikssonR D Goldman
Aug 19, 1991·International Journal of Cancer. Journal International Du Cancer·M R YoungJ M Bagash
Jan 1, 1991·Methods in Enzymology·D G HardieA T Sim
Jan 21, 1992·International Journal of Cancer. Journal International Du Cancer·J Smolle, I A Grimstad
Jul 30, 1993·International Journal of Cancer. Journal International Du Cancer·M R YoungG D Maier

❮ Previous
Next ❯

Citations

Mar 21, 2007·Clinical & Experimental Metastasis·M Rita I YoungTerry A Day
Apr 11, 1997·European Journal of Pharmacology·V Niggli, H Keller
Oct 3, 1999·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·S GabelM r Young
Jul 13, 2001·Cancer Letters·A H Schönthal
Mar 21, 2001·Cellular Signalling·E Sontag
Sep 6, 2000·American Journal of Respiratory Cell and Molecular Biology·T M HongC W Wu
Jan 25, 2005·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Natsuko NakadaEi Kawahara
Nov 24, 1999·The Journal of Biological Chemistry·M CondrescuJ P Reeves
Mar 26, 2003·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Susann Schweiger, Rainer Schneider
Nov 8, 2005·The Journal of Experimental Biology·I R AresL M Botana

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.