Regulation of cytoskeletal protein organization and expression in human granulosa cells in response to gonadotropin treatment

Endocrinology
A Ben-Ze'ev, A Amsterdam

Abstract

The organization and expression of the major cytoskeletal protein systems and of several cytoskeleton-associated proteins were analyzed in human granulosa cells obtained from preovulatory follicles in the course of an in vitro fertilization program. The cells were cultured in medium containing 5% fetal calf serum in the absence or presence of gonadotropins. Within several hours after the addition of hCG to cultured human granulosa cells, the cells acquired a rounded and aggregated morphology with numerous thin and long processes. Cells cultured without the gonadotropin had a flat and extended morphology on the substrate, with a well developed stress fiber system and numerous large vinculin-containing adhesion plaques. The [35S]methionine-labeled protein pattern of hCG-treated cells obtained by two-dimensional isoelectric focusing and sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed a 7- to 10-fold decrease in the synthesis of vinculin and the nonmuscle tropomyosin isoforms, and a 2- to 4-fold decrease in the synthesis of alpha-actinin and actin, without a comparable change in alpha- and beta-tubulin synthesis. In the absence of gonadotropins, human granulosa cells synthesized both the intermediate filament (IF...Continue Reading

Citations

Feb 1, 1994·Microscopy Research and Technique·A Amsterdam, D Aharoni
May 1, 1995·Histochemistry and Cell Biology·A M RickenP R Huber
Jan 30, 2003·Acta histochemica·Elena Marettová, Milan Maretta
May 6, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·A BonnetG Tosser-Klopp
May 5, 2016·Medical Hypotheses·Mariano BizzarriArturo Bevilacqua
Jan 1, 1997·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·F S Khan-DawoodM Y Dawood

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