Regulation of Discrete Functional Responses by Syk and Src Family Tyrosine Kinases in Human Neutrophils

Journal of Immunology Research
Thornin EarPatrick P McDonald

Abstract

Neutrophils play a critical role in innate immunity and also influence adaptive immune responses. This occurs in good part through their production of inflammatory and immunomodulatory cytokines, in conjunction with their prolonged survival at inflamed foci. While a picture of the signaling machinery underlying these neutrophil responses is now emerging, much remains to be uncovered. In this study, we report that neutrophils constitutively express various Src family isoforms (STKs), as well as Syk, and that inhibition of these protein tyrosine kinases selectively hinders inflammatory cytokine generation by acting posttranscriptionally. Accordingly, STK or Syk inhibition decreases the phosphorylation of signaling intermediates (e.g., eIF-4E, S6K, and MNK1) involved in translational control. By contrast, delayed apoptosis appears to be independent of either STKs or Syk. Our data therefore significantly extend our understanding of which neutrophil responses are governed by STKs and Syk and pinpoint some signaling intermediates that are likely involved. In view of the foremost role of neutrophils in several chronic inflammatory conditions, our findings identify potential molecular targets that could be exploited for future therapeu...Continue Reading

References

Jul 22, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Thornin EarPatrick P McDonald
Oct 17, 2006·Oncogene·Y MamaneN Sonenberg
Dec 2, 2006·Journal of Leukocyte Biology·Laurie E KilpatrickHelen M Korchak
Sep 11, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Carl F FortinPatrick P McDonald
Dec 26, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Alexandre CloutierPatrick P McDonald
Mar 5, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Thornin EarPatrick P McDonald
Apr 7, 2011·European Journal of Immunology·Carl F FortinPatrick P McDonald
Jul 26, 2011·Nature Reviews. Immunology·Alberto MantovaniSébastien Jaillon
Mar 14, 2012·Blood·Krisztina FutosiAttila Mócsai
Feb 13, 2013·Journal of Leukocyte Biology·Carl F FortinPatrick P McDonald
Jul 5, 2013·The Journal of Experimental Medicine·Attila Mócsai
Sep 3, 2013·International Immunopharmacology·Krisztina FutosiAttila Mócsai
Sep 17, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Thomas Z MayerPatrick P McDonald
Nov 7, 2014·Frontiers in Immunology·Cristina TecchioMarco A Cassatella
Aug 26, 2016·Immunological Reviews·Krisztina Futosi, Attila Mócsai

❮ Previous
Next ❯

Methods Mentioned

BETA
FACS
PCR
ELISA

Software Mentioned

Prism
CytExpert
GraphPad

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Related Papers

Journal of Leukocyte Biology
Carl F FortinPatrick P McDonald
The Journal of Immunology : Official Journal of the American Association of Immunologists
Stéphanie Sylvain-PrévostPatrick P McDonald
Experimental Cell Research
C L Abram, S A Courtneidge
© 2022 Meta ULC. All rights reserved