Regulation of DNA Double Strand Breaks Processing: Focus on Barriers

Frontiers in Molecular Biosciences
Federica MariniAchille Pellicioli

Abstract

In all the eukaryotic cells, nucleolytic processing (resection) of a double strand DNA break (DSB) is a key step to channel the repair of the lesion toward the homologous recombination, at the expenses of the non-homologous end joining (NHEJ). The coordinated action of several nucleases and helicases generates 3' single strand (ss) DNA, which is covered by RPA and recombination factors. Molecular details of the process have been first dissected in the model organism Saccharomyces cerevisiae. When DSB ends are occupied by KU, a central component of the NHEJ, the Mre11-Rad50-Xrs2 (MRX) nuclease complex (MRN in human), aided by the associated factors Sae2 (CTIP in human), initiates the resection process, inducing a nick close to the DSB ends. Then, starting from the nick, the nucleases Mre11, Exo1, Dna2, in cooperation with Sgs1 helicase (BLM in human), degrade DNA strand in both the directions, creating the 3' ssDNA filament. Multiple levels of regulation of the break processing ensure faithful DSB repair, preventing chromosome rearrangements, and genome instability. Here we review the DSB resection process and its regulation in the context of chromatin. Particularly, we focus on proteins that limit DSB resection, acting as physi...Continue Reading

References

Feb 23, 1999·Biochemistry·D W ChanS P Lees-Miller
May 23, 2003·Cold Spring Harbor Symposia on Quantitative Biology·S E LeeJ E Haber
Dec 21, 2006·Molecular and Cellular Biology·Eun Yong ShimSang Eun Lee
Aug 6, 2008·The Journal of Cell Biology·Lisa PostowHironori Funabiki
Aug 22, 2008·Nature·Pablo HuertasStephen P Jackson
Feb 10, 2009·The Journal of Biological Chemistry·Pablo Huertas, Stephen P Jackson
Aug 10, 2010·Annual Review of Genetics·Wolf-Dietrich HeyerJie Liu
Oct 13, 2010·Proceedings of the National Academy of Sciences of the United States of America·Danny M ChouStephen J Elledge
Aug 31, 2011·Molecular and Cellular Biology·Steven S FosterJohn H J Petrini
Jan 24, 2012·Nature Structural & Molecular Biology·Lin Feng, Junjie Chen
Feb 22, 2012·Nature Structural & Molecular Biology·Tibor PankotaiEvi Soutoglou
Jun 23, 2012·Nature·Sofia FranciaFabrizio d'Adda di Fagagna
Mar 19, 2013·Cell·Brendan D Price, Alan D D'Andrea
Jun 4, 2013·Nature Structural & Molecular Biology·Nicholas L AdkinsCraig L Peterson
Jun 14, 2013·Nature·Amélie Fradet-TurcotteDaniel Durocher
Oct 8, 2013·Trends in Cell Biology·Michal Zimmermann, Titia de Lange
Dec 7, 2013·Cellular and Molecular Life Sciences : CMLS·Kee-Beom KimSang-Beom Seo
Dec 12, 2013·Nature Reviews. Molecular Cell Biology·Stephanie Panier, Simon J Boulton
Feb 26, 2014·The Journal of Biological Chemistry·Lisa E HangXiaolan Zhao
Sep 5, 2014·Nature·Havva KeskinFrancesca Storici
Apr 30, 2015·Cell Reports·Jessica S BrownStephen P Jackson
Oct 23, 2015·Nucleic Acids Research·Diego DibitettoAchille Pellicioli
Jan 18, 2016·Molecular Cell·Ján TkáčDaniel Durocher
Apr 22, 2016·Critical Reviews in Biochemistry and Molecular Biology·Lorraine S Symington
May 31, 2016·Nature Structural & Molecular Biology·Ruth M DenshamJoanna R Morris
Jun 28, 2016·Nature Structural & Molecular Biology·Fena OchsClaudia Lukas
Oct 30, 2016·Molecular and Cellular Biology·Lei LiRoseline Godbout
Sep 20, 2016·Nature Communications·Pauline ChanutPatrick Calsou

❮ Previous
Next ❯

Citations

Jul 4, 2020·Essays in Biochemistry·Ruth F Ketley, Monika Gullerova
Jun 25, 2020·Nature Communications·Matteo FerrariAchille Pellicioli
Sep 17, 2020·International Journal of Molecular Sciences·Samuele LodovichiAlvaro Galli
Jan 11, 2020·Frontiers in Oncology·Anika Trenner, Alessandro A Sartori
Mar 7, 2020·International Journal of Molecular Sciences·Giulia Maria NavaFederico Lazzaro
Jul 16, 2020·Cells·Antonio Carusillo, Claudio Mussolino
Jan 12, 2021·Frontiers in Molecular Biosciences·Kendal Prill, John F Dawson
Nov 17, 2020·Frontiers in Molecular Biosciences·Rick A C M BoonenHaico van Attikum
Sep 21, 2020·Seminars in Cell & Developmental Biology·Nour L MozaffariAlessandro A Sartori
Apr 11, 2021·Nature Cell Biology·Aline Marnef, Gaëlle Legube
Aug 24, 2021·Frontiers in Cell and Developmental Biology·Marilina RaicesJudith L Yanowitz
Jul 10, 2021·Trends in Genetics : TIG·Charlotte AudoynaudSarah Lambert

❮ Previous
Next ❯

Methods Mentioned

BETA
neddylation
sumoylation
acetylation
ubiquitination

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.