PMID: 7029525Aug 1, 1981Paper

Regulation of DNA replication: "target" determinant of the replication control elements of plasmid R6-5 lies within a control element gene

Proceedings of the National Academy of Sciences of the United States of America
H DanbaraK N Timmis

Abstract

The replication control system of plasmid R6-5 has been investigated by characterization of high-copy-number mutant miniplasmids, development of an in vivo assay for the site of action or "target" of the replication control elements, and sequence analysis of the replication control regions of the wild-type plasmid and two copy-number mutant derivatives. These and other experiments have shown that three plasmid determinants--copA/incA, copB, and copT--are involved in DNA replication control. The products of the copB and copA/incA genes, a 9500-dalton basic polypeptide and either a 7200-dalton basic polypeptide or a short untranslated RNA molecule, respectively, are negative-acting elements that interact with the third element, their target, the copT DNA sequence, or its product to regulate the frequency of initiation of plasmid replication. The location of copT within the copA/incA gene and 1600 base pairs upstream from the origin of replication indicates that regulation is effected at a preinitiation stage of replication, such as the production of a primer or other initiation factor.

References

Jan 1, 1979·Annual Review of Genetics·M Rosenberg, D Court
Feb 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·A M Maxam, W Gilbert
Jan 1, 1979·Cold Spring Harbor Symposia on Quantitative Biology·K BackmanS Yanofsky
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May 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·T Itoh, J Tomizawa

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Citations

Jan 1, 1982·Molecular & General Genetics : MGG·J Light, S Molin
Jan 1, 1984·Molecular & General Genetics : MGG·B R BaumstarkJ R Scott
Oct 1, 1986·Molecular & General Genetics : MGG·K A ArmstrongE Ohtsubo
Sep 30, 1986·Biochemical and Biophysical Research Communications·H YoshimotoM Yoshikawa
Jan 1, 1984·Microbiology and Immunology·T YoshidaM Yoshikawa
Oct 4, 2005·Plasmid·Kurt Nordström
Jan 1, 1990·Critical Reviews in Biochemistry and Molecular Biology·K M Takayama, M Inouye
Jan 1, 1987·Microbiology and Immunology·A MochizukiR Nakaya

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