PMID: 11901220Mar 20, 2002Paper

Regulation of dopamine D(1) receptor trafficking by protein kinase A-dependent phosphorylation

Molecular Pharmacology
John N MasonKim A Neve

Abstract

The aim of this study was to use pharmacological inhibition of protein kinase A and mutation of potential protein kinase A phosphorylation sites to determine the role of protein kinase A-catalyzed phosphorylation of the dopamine D(1) receptor in agonist-stimulated desensitization and internalization of the receptor. To facilitate purification and imaging of the D(1) receptor, we attached a polyhistidine tag to the amino terminus and enhanced green fluorescent protein to the carboxyl terminus of the receptor (D(1)-EGFP). D(1)-EGFP was similar to the untagged D(1) receptor in terms of affinity for agonist and antagonist ligands, coupling to G proteins, and stimulation of cyclic AMP accumulation. D(1)-EGFP and two mutants in which either Thr268 or Ser380 was replaced with Ala were stably expressed in NS20Y neuroblastoma cells. Pretreatment with the protein kinase A inhibitor H-89 (N-[2-(p-bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide) or substitution of Ala for Thr268 reduced agonist-stimulated phosphorylation of the receptor and resulted in diminished trafficking of the receptor to the perinuclear region of the cell. Substitution of Ala for Thr268 had no effect, however, on agonist-induced receptor sequestration or desensit...Continue Reading

References

May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J L BenovicR J Lefkowitz
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M MemoI Hanbauer
Mar 15, 1994·European Journal of Pharmacology·G Y NgB F O'Dowd
Mar 1, 1995·The Journal of Cell Biology·B F RoettgerL J Miller
Jul 1, 1997·Physiological Reviews·S MukherjeeF R Maxfield
May 23, 1998·Annual Review of Pharmacology and Toxicology·J G Krupnick, J L Benovic
Feb 23, 1999·Advances in Experimental Medicine and Biology·H M SadeghiM Birnbaumer
Feb 13, 2001·Trends in Pharmacological Sciences·P TsaoM von Zastrow

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Citations

Oct 12, 2005·Pflügers Archiv : European journal of physiology·Hjalmar BrismarUlla Holtbäck
Mar 12, 2003·Life Sciences·Paula A Witt-EnderbyMelissa A Melan
Feb 22, 2008·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Elizabeth B RexDavid R Sibley
Dec 1, 2004·Kidney International·Peiying YuPedro A Jose
Mar 19, 2005·Journal of Neurochemistry·Tara A MaceyKim A Neve
Dec 15, 2004·Journal of Pharmacological and Toxicological Methods·K H S ArunP Ramarao
Feb 7, 2006·Pharmacology & Therapeutics·M J Hurley, P Jenner
Jul 28, 2011·Neuron·Sarah J KotowskiMark von Zastrow
May 29, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Sónia FragaPatrício Soares-da-Silva
Dec 24, 2013·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Ning WangYun Wang
Sep 7, 2004·The Journal of Biological Chemistry·Yoon Namkung, David R Sibley
Jun 30, 2005·Archives of Insect Biochemistry and Physiology·Julie A MustardAlison R Mercer
Oct 26, 2010·Journal of Neurochemistry·Michele L Rankin, David R Sibley
Dec 13, 2005·Molecular Pharmacology·Michele L RankinDavid R Sibley
Dec 13, 2005·Molecular Pharmacology·Kim A Neve
Aug 19, 2007·Molecular Pharmacology·Michael M C KongSusan R George
Nov 26, 2009·Cellular Signalling·Anneleen SpoorenKathleen Van Craenenbroeck

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