PMID: 8965650Mar 1, 1996Paper

Regulation of dopamine D2 receptor affinity by cholecystokinin octapeptide in fibroblast cells cotransfected with human CCKB and D2L receptor cDNAs

Brain Research. Molecular Brain Research
S DasguptaK Fuxe

Abstract

Alteration in dopamine (DA) and/or cholecystokinin (CCK) transmission in the CNS may be of relevance for schizophrenia. Previous findings in striatal membranes give indications of a modulation of DA D2 receptor affinity by CCKB receptor activation. In the present study receptor binding studies were performed in a mouse fibroblast cell line (L-hD2l/CCK), expressing both human D2 receptors (long form, D2L) and human CCKB receptors, and binding sites for [3H]CCK-8S (sulfated CCK octapeptide), the D2 agonist [3H]NPA and the D2 antagonist [3H]raclopride were found and characterized in saturation and competition experiments. 1 nM of CCK-8 caused a significant 38% increase in the KD value of the D2 agonist [3H]NPA binding sites in the L-hD2l/CCK cell membranes. This change was blocked by the CCKB receptor antagonist PD 134308 (50 nM). Furthermore, 1 nM of CCK-8 increased the KD value of the D2 antagonist [3H]raclopride binding sites by 34% (P < 0.05) in the L-hD2l/CCK cell membranes. Control cells (L-hD2l cells) expressing D2L receptors showed no specific [3H]CCK-8S binding sites and no modulation by CCK-8 of the D2L receptors. These findings indicate a modulation of the D2L receptor affinity by activation of the CCKB receptor also wh...Continue Reading

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Citations

Dec 5, 2009·Journal of Molecular Neuroscience : MN·Alexander O Tarakanov, Kjell G Fuxe
Jun 19, 2003·Neuroscience Letters·Mohamed A ElwanNorio Sakuragawa
May 19, 2011·Clinical Neuropharmacology·Francisco López-Muñoz, Cecilio Álamo
Apr 12, 2003·Pharmacology & Toxicology·Paul MicevychKevin Sinchak
Jun 21, 2012·Journal of Receptor and Signal Transduction Research·Alexander O TarakanovDasiel O Borroto-Escuela
May 21, 1999·Journal of Neuroscience Research·M A ElwanN Sakuragawa

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