Regulation of embryonic development and apoptotic-related gene expression by brain-derived neurotrophic factor in two different culture conditions in ovine

Theriogenology
Amir Hossein Abazari-KiaMohammad Salehi

Abstract

In the present study, we aimed to evaluate effects of brain-derived neurotrophic factor (BDNF) which is a member of neurotrophic factor family on developmental competence of oocytes in sheep. In vitro maturation was performed in presence of various concentrations (0, 10, and 100 ng/mL) of BDNF. Meiotic maturation, levels of intracellular glutathione, embryonic developmental potential after parthenogenetic activation, number of total and apoptotic cells in blastocysts, and expression of Bax and Bcl-2 genes in blastocyst cells were determined. Under unstressed condition, while at 100 ng/mL concentration, BDNF increased the IVM rate; an increase of glutathione level was observed at 10 ng/mL concentration. Moreover, when BDNF-treated oocytes were used for parthenogenetic activation, more blastocyst at both 10 and 100 ng/mL levels was obtained in comparison with the untreated group. Under heat stress (HS), the blastocyst rate was dramatically reduced in untreated oocytes compared to that obtained from 10 ng/mL BDNF groups. Total cell number in blastocysts was not affected by the treatment groups. The mean of Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-positive nuclei in blastocysts was not influenced by addi...Continue Reading

References

Apr 18, 1995·Brain Research. Developmental Brain Research·T KuboH Hatanaka
Nov 1, 1994·Journal of Neurobiology·M Barbacid
Oct 8, 1999·Cell Death and Differentiation·M D FeliciM Piacentini
May 23, 2001·Leukemia·P P RuvoloW S May
Feb 12, 2002·The Journal of Clinical Endocrinology and Metabolism·David B SeiferCheryl F Dreyfus
Apr 12, 2002·Animal Reproduction Science·Ming Yuan Yang, Rajadurai Rajamahendran
Sep 19, 2002·Molecular Reproduction and Development·C E KriningerP J Hansen
Dec 6, 2002·Microscopy Research and Technique·Barbara BarboniNicola Bernabò
Mar 27, 2003·Molecular Reproduction and Development·S Sirisathien, B G Brackett
Jun 6, 2003·Cytokine & Growth Factor Reviews·Shahrooz Rabizadeh, Dale E Bredesen
Nov 5, 2003·Journal of Cellular and Molecular Medicine·Alexandrina Burlacu
Apr 23, 2004·Nature·Tomohiro KonoHidehiko Ogawa
Feb 8, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Z Roth, P J Hansen
Mar 31, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·S J Martins da SilvaR A Anderson
Jun 22, 2005·Proceedings of the National Academy of Sciences of the United States of America·Kazuhiro KawamuraAaron J W Hsueh
Aug 22, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·Eugine LeeWoo Suk Hwang
Oct 28, 2008·Molecular Reproduction and Development·S Clay IsomEdmund B Rucker
Apr 18, 2009·Zygote : the Biology of Gametes and Early Embryos·So Gun HongByeong Chun Lee
Nov 17, 2009·Journal of Assisted Reproduction and Genetics·Mahdi ZhandiAhmad Zare-Shahneh
Feb 8, 2011·Theriogenology·Sarah D FieldsAlan D Ealy
Apr 9, 2011·Cellular Reprogramming·M H Nasr-EsfahaniH Vojgani
Dec 21, 2013·Journal of Assisted Reproduction and Genetics·Amir Hossein Abazari-KiaMohammad Salehi
Jan 15, 2014·Journal of Assisted Reproduction and Genetics·Maryam Dehghani-MohammadabadiZahra Nourmohammadi

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Related Feeds

Brain Injury & Trauma

brain injury after impact to the head is due to both immediate mechanical effects and delayed responses of neural tissues.

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis