Regulation of endothelial cell branching morphogenesis by endogenous chemokine stromal-derived factor-1.

Blood
Ombretta SalvucciGiovanna Tosato

Abstract

The chemokine stromal-derived factor-1 (SDF-1) and its unique receptor, CXCR4, are required for normal cardiovascular development, but a critical role for SDF-1 in postnatal vascular remodeling and the mechanisms underlying SDF-1/CXCR-4 vasculogenesis are unclear. Here we show that SDF-1 is expressed by the vascular endothelium from selected healthy and tumor tissues. In vitro, primary endothelial cells constitutively express SDF-1 that is detected in the cytoplasm, on the cell surface, and in the culture supernatant. Vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) increase SDF-1 expression in endothelial cells. In functional studies, pertussis toxin and antibodies to SDF-1 or CXCR-4 disrupt extracellular matrix-dependent endothelial cell tube formation in vitro. This morphogenic process is associated with time-dependent modulation of surface CXCR-4 expression that changes from being diffuse to being polarized and subsequently lost. In vivo, pertussis toxin and neutralizing antibodies directed at SDF-1 inhibit growth factor-dependent neovascularization. These results indicate that SDF-1/CXCR-4 identifies VEGF- and bFGF-regulated autocrine signaling systems that are essential regulators of end...Continue Reading

References

Apr 1, 1991·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·D S GrantH K Kleinman
Jan 1, 1989·Annual Review of Cell Biology·H R Pelham
Feb 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·H K KleinmanL Luckenbill-Edds
Dec 11, 1980·Nature·J Folkman, C Haudenschild
Sep 1, 1983·In Vitro·P B GordonV B Hatcher
Jul 1, 1995·The Journal of Experimental Medicine·A L AngiolilloG Tosato
Sep 1, 1996·The Journal of Experimental Medicine·C C BleulT A Springer
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·Q MaT A Springer
May 13, 1999·Proceedings of the National Academy of Sciences of the United States of America·K KawabataT Nagasawa
Nov 7, 1999·The American Journal of Pathology·J L PablosF Arenzana-Seisdedos
Jan 5, 2000·The Journal of Clinical Investigation·D Y JoM A Moore
Mar 31, 2000·Nature Medicine·P Carmeliet
Apr 20, 2000·Journal of the National Cancer Institute·A CorcioneV Pistoia
May 10, 2000·Nature Medicine·M C PoznanskyD T Scadden
Sep 23, 2000·Nature·G D YancopoulosJ Holash
Sep 23, 2000·Nature·P Carmeliet, R K Jain
Dec 6, 2000·The Journal of Clinical Investigation·T PonomaryovT Lapidot

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Citations

Aug 19, 2008·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Pinghong LaiShibo Tang
Aug 6, 2005·Journal of Gastroenterology·Masumi AkimotoKatsuko Yamashita
Jan 1, 2014·Journal of Neural Transmission·Dirk M HermannAurel Popa-Wagner
Dec 14, 2005·Breast Cancer Research and Treatment·Ombretta SalvucciMark Basik
Sep 29, 2006·Cancer Metastasis Reviews·Gera Neufeld, Ofra Kessler
Dec 14, 2006·Cancer Metastasis Reviews·Jianhua WangRussell S Taichman
Nov 24, 2006·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Yun YangYin Zhao
Jan 5, 2008·International Journal of Gastrointestinal Cancer·Daniel D BilladeauRaul Urrutia
Oct 29, 2003·Current Opinion in Biotechnology·Hynda K KleinmanMatthew P Hoffman
May 12, 2005·Trends in Cardiovascular Medicine·Daniel J Ceradini, Geoffrey C Gurtner
Aug 8, 2008·Expert Reviews in Molecular Medicine·Joshua C AndersonCandece L Gladson
Mar 7, 2007·The Journal of Experimental Medicine·Jessy DeshaneAnupam Agarwal
Oct 13, 2006·Antioxidants & Redox Signaling·Nikolaos G Frangogiannis
Nov 25, 2011·Carcinogenesis·W Andrew YeudallSumitra Deb
Feb 8, 2007·Journal of the National Cancer Institute·Takayuki NakayamaGiovanna Tosato
May 15, 2007·Transplantation·Caroline Fréguin-BouillandDidier Plissonnier
Apr 29, 2006·Artificial Organs·A Ruchan AkarUmit Ozyurda
Mar 5, 2005·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Kohei MiuraBosco M C Chan
Nov 21, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Parvaneh RafieeDavid G Binion
Sep 4, 2010·International Journal of Rheumatology·Tracy FrechAllen Sawitzke
Nov 8, 2011·Reproductive Biology and Endocrinology : RB&E·Ryan L AshleyThomas R Hansen
Sep 20, 2008·PLoS Computational Biology·Roeland M H MerksJames A Glazier

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