Regulation of endothelial Na(+)-K(+)-ATPase activity by cAMP

Biochemical and Biophysical Research Communications
M L GruwelJ Schrader

Abstract

Using an in vitro cell system and Cs+ NMR techniques we were able to show that porcine aortic endothelial cells (PAEC) reduce their Na(+)-K(+)-ATPase activity upon an increase in intracellular cAMP. Reduction in the pump rate was due to phosphorylation of the alpha-subunit of the ATPase as shown by immunoprecipitation. Apart from a pump inhibiton using 8-Br-cAMP and IBMX, we were also able to show that changes in the Na(+)-K(+)-ATPase activity could be mediated by the adenosine-A2 and prostaglandin receptor agonists 5'-N-Ethylcarboxamidoadenosine and Iloprost, respectively. Parallel to a decrease in pump activity we also observed a decrease in intracellular Cs+, indicating opening of K+ channels.

References

Dec 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·A M BertorelloP Greengard
Oct 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·B MeisterP Greengard
Jan 2, 1989·FEBS Letters·J L AllisG K Radda
Feb 1, 1986·The American Journal of Physiology·J W Mills, M Lubin
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May 1, 1997·The American Journal of Physiology·P A SchornackJ J Ackerman

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Citations

Mar 17, 2005·NMR in Biomedicine·James GoodmanJoseph J H Ackerman
Dec 22, 2007·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·James A GoodmanJeffrey J Neil

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