PMID: 7526698Nov 1, 1994Paper

Regulation of endothelial nitric oxide synthase mRNA, protein, and activity during cell growth

The American Journal of Physiology
J F ArnalD G Harrison

Abstract

Cell growth influences the expression of several important tissue-specific functions. We sought to examine the effect of cell proliferation on nitric oxide (NO) synthase gene expression in cultured aortic bovine endothelial cells. Western and Northern blot analysis revealed three- and sixfold increases in NO synthase protein and mRNA, respectively, in growing compared with growth-arrested cells. The release of nitrogen oxides was also increased in proliferating cells compared with growth-arrested cells, as was the NO synthase activity assessed by L-arginine/L-citrulline conversion. Neither NO synthase inhibitors nor superoxide dismutase affected proliferation or thymidine incorporation, suggesting that increased NO release had no effect on endothelial cell growth. In conclusion, these studies demonstrate that expression of endothelial cell NO synthase is markedly increased in proliferating compared with quiescent nongrowing cells. The mechanisms underlying this and its physiological consequences remain to be defined.

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Citations

Mar 1, 1996·Clinical and Experimental Pharmacology & Physiology·D G HarrisonR C Venema
Jul 1, 1996·Journal of Cardiovascular Pharmacology·J B MichelG Chatellier
Nov 15, 1997·The American Journal of Physiology·M ChallahJ B Michel
Jul 1, 1997·Clinical and Experimental Hypertension : CHE·O CosterousseF Alhenc-Gelas
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Dec 10, 2002·Antioxidants & Redox Signaling·Toyoaki Murohara, Takayuki Asahara
Dec 6, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Sharon C TaiPhilip A Marsden
Aug 13, 2004·Vascular and Endovascular Surgery·Joseph L UnthankMichael C Dalsing
Oct 26, 2006·The Journal of Physiological Sciences : JPS·Kazuyoshi NakamuraManabu Kubokawa
May 12, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ru-Jeng TengGirija G Konduri
Feb 1, 1996·Clinical and Experimental Hypertension : CHE·S Nadaud, F Soubrier
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