PMID: 2117475Jul 1, 1990Paper

Regulation of endothelial tissue plasminogen activator and plasminogen activator inhibitor type 1 synthesis by diacylglycerol, phorbol ester, and thrombin

Blut
J Grulich-Henn, G Müller-Berghaus

Abstract

The influence of diacylglycerols, which are physiological activators of protein kinase C, on the production of tissue-type plasminogen activator (tPA) and plasminogen activator inhibitor type 1 (PAI-1) by human umbilical vein endothelial cells (HUVEC) was studied in order to gain insight into the regulation of fibrinolysis by these cells. 1,2-dioctanoyl-sn-glycerol (diC8) stimulated tPA production in a dose- and time-dependent manner. The tPA antigen in cell supernatants increased from 0.9 ng/10(6) cells in unstimulated cells to 12.4 ng (10(6) cells after incubation with 400 microM diC8 for 24 hours. In contrast, PAI-1 production was not influenced by diC8, whereas phorbol 12-myristate 13-acetate (PMA) or thrombin stimulated both, tPA and PAI-1 production by HUVEC. Staurosporine and H7, which are inhibitors of protein kinase C, inhibited tPA synthesis by HUVEC. The degree of inhibition was dependent on the agonist used. While diC8-induced tPA production was inhibited to more than 80% by H7 (10 microM) and staurosporine (10 nM), higher doses of inhibitors were required to inhibit thrombin- and PMA-induced tPA production. Thrombin-induced PAI-1 production was inhibited to more than 80% by H7 (10 microM) and to about 50% by stauro...Continue Reading

References

Jan 1, 1986·The Journal of Clinical Investigation·T D Gelehrter, R Sznycer-Laszuk
Feb 15, 1986·Thrombosis Research·C KorningerB R Binder
Aug 1, 1986·The Journal of Clinical Investigation·M P BevilacquaD J Loskutoff
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J G EbelingJ E Niedel
Apr 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·A M JettenR M Bell
Jul 1, 1987·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·V W van HinsberghD C Rijken
Oct 14, 1987·Biochemical and Biophysical Research Communications·W W Lo, T P Fan
Oct 15, 1985·The Biochemical Journal·K T PreissnerG Müller-Berghaus
Dec 1, 1982·The Journal of Cell Biology·J L GrossD B Rifkin
Nov 30, 1984·Biochemical and Biophysical Research Communications·S Kawamoto, H Hidaka
Dec 1, 1984·The Journal of Clinical Investigation·E G LevinL A Harker

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Citations

Mar 1, 1995·European Journal of Obstetrics, Gynecology, and Reproductive Biology·C SchubringG Müller-Berghaus
Dec 4, 1992·Annals of the New York Academy of Sciences·V W van Hinsbergh
Jun 1, 1991·European Journal of Clinical Chemistry and Clinical Biochemistry : Journal of the Forum of European Clinical Chemistry Societies·J W van WerschF A Lustermans
Dec 1, 1996·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·J Müller, T Yoshida
Jan 18, 2006·Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology·Guoping M MaGarry X Shen
Sep 1, 1992·Annals of Vascular Surgery·M WelchM G Walker
Jun 19, 2004·International Journal of Legal Medicine·Masataka TakamiyaYasuhiro Aoki
Feb 9, 2000·American Journal of Physiology. Endocrinology and Metabolism·S PuriR R MacGregor
Apr 6, 2000·American Journal of Physiology. Endocrinology and Metabolism·S RenG X Shen

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