PMID: 8603098Mar 27, 1996Paper

Regulation of energy metabolism by interleukin-1beta, but not by interleukin-6, is mediated by nitric oxide in primary cultured rat hepatocytes

Biochimica Et Biophysica Acta
H KitadeTadayoshi Okumura

Abstract

The effects of inflammatory cytokines (interleukin-1beta, interleukin-6, and tumor necrosis factor-alpha) on energy metabolism were studied in primary cultured rat hepatocytes. Adenine nucleotide (ATP, ADP, and AMP) content, lactate production, the ketone body ratio (acetoacetate/beta-hydroxybutyrate) reflecting the liver mitochondrial redox state (NAD+/NADH), and nitric oxide formation were measured. Insulin increased ATP content in hepatocytes and had a maximal effect after 8-12 h of culture. Both interleukin-1beta and interleukin-6, but not tumor necrosis factor-alpha, significantly inhibited the ATP increase time- and dose-dependently. Interleukin-1beta and interleukin-6 also stimulated lactate production. During the same period, interleukin-1beta but not interleukin-6 decreased the ketone body ratio. Furthermore, interleukin-1beta markedly stimulated nitric oxide formation in hepatocytes, and this increase was blocked by NG-monomethyl-L-arginine (a nitric oxide synthase inhibitor) and by interleukin-1 receptor antagonist. NG-monomethyl-L-arginine reversed inhibition of the ATP increase, decrease in the ketone body ratio, and increase in lactate production, which were induced by interleukin-1beta. Interleukin-1 receptor ant...Continue Reading

References

Feb 1, 1991·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·T AndusW Gerok
Dec 15, 1987·Biochemical Pharmacology·S A el-Aleem, H Schulz
Jan 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·D A GellerT R Billiar
May 24, 1994·Proceedings of the National Academy of Sciences of the United States of America·G S HotamisligilB M Spiegelman
Jan 1, 1994·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J A Spitzer

❮ Previous
Next ❯

Citations

Jan 26, 2000·Journal of Pediatric Surgery·C RomeoA Pierro
Apr 1, 2000·Clinical and Experimental Pharmacology & Physiology·S SatoiH Takahashi
Apr 1, 2009·BMC Cell Biology·Paulina WegrzynJolanta Jura
Aug 23, 2007·JPEN. Journal of Parenteral and Enteral Nutrition·Kosuke MatsuiTadayoshi Okumura
Apr 2, 1998·The Journal of Laboratory and Clinical Medicine·S SatoiH Takahashi
Oct 7, 2004·Journal of Hepatology·Céline FilippiJohn N Plevris
Oct 26, 2000·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·T InoueT Okumura
May 23, 1998·In Vitro Cellular & Developmental Biology. Animal·T KamiyaY Kamiyama
May 8, 2013·Hepatology Research : the Official Journal of the Japan Society of Hepatology·Emi YoshigaiMikio Nishizawa
Mar 13, 2020·Cellular & Molecular Biology Letters·Mohsen KeshavarzFarhad Rezaei
Feb 16, 2021·Frontiers in Endocrinology·Christopher R Shepard
May 13, 1999·Biochimica Et Biophysica Acta·G C Brown

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.