Regulation of enzyme activities in carnivorous pitcher plants of the genus Nepenthes

Planta
Michaela SaganováAndrej Pavlovič

Abstract

Nepenthes regulates enzyme activities by sensing stimuli from the insect prey. Protein is the best inductor mimicking the presence of an insect prey. Carnivorous plants of the genus Nepenthes have evolved passive pitcher traps for prey capture. In this study, we investigated the ability of chemical signals from a prey (chitin, protein, and ammonium) to induce transcription and synthesis of digestive enzymes in Nepenthes × Mixta. We used real-time PCR and specific antibodies generated against the aspartic proteases nepenthesins, and type III and type IV chitinases to investigate the induction of digestive enzyme synthesis in response to different chemical stimuli from the prey. Transcription of nepenthesins was strongly induced by ammonium, protein and live prey; chitin induced transcription only very slightly. This is in accordance with the amount of released enzyme and proteolytic activity in the digestive fluid. Although transcription of type III chitinase was induced by all investigated stimuli, a significant accumulation of the enzyme in the digestive fluid was found mainly after protein and live prey addition. Protein and live prey were also the best inducers for accumulation of type IV chitinase in the digestive fluid. Al...Continue Reading

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Citations

May 24, 2019·Journal of Experimental Botany·Andrej Pavlovič, Axel Mithöfer
May 6, 2020·Plants·Zelalem Eshetu BekaluAnd Henrik Brinch-Pedersen
Mar 29, 2020·Journal of Experimental Botany·Ondřej KocábAndrej Pavlovič
Jul 22, 2021·AoB Plants·Kadeem J Gilbert, Tanya Renner

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Methods Mentioned

BETA
electrophoresis
PCR
ELISA
X-ray
light-microscopy
transmission electron microscopy
deamination

Software Mentioned

Excel
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