Regulation of enzymes of the 3,5-xylenol-degradative pathway in Pseudomonas putida: evidence for a plasmid.

Journal of Bacteriology
D J Hopper, P D Kemp

Abstract

Constitutive synthesis of enzymes responsible for methyl group oxidation in 3,5-xylenol degradation and an associated p-cresol methylhydroxylase in Pseudomonas putida NCIB 9869 was shown by their retention at high specific activities in cells transferred from 3,5-xylenol medium to glutamate medium. The specific activities of other enzymes of the 3,5-xylenol pathway declined upon removal of aromatic substrate, consistent with their inducible control. Specific activities of the methyl-oxidizing enzymes showed an eventual decline concomitant with a decrease in the fraction of bacteria capable of growth with 3,5-xylenol; a simultaneous loss of the ability to grow with m-hydroxybenzoate was also observed. The property of 3,5-xylenol utilization could be transferred to another strain of P. putida. It is proposed that enzymes of the 3,5-xylenol pathway and those for conversion of p-cresol to p-hydroxybenzoate are plasmid encoded, that the early methyl-oxidizing enzymes are expressed constitutively, and that the later enzymes are inducible.

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Citations

Mar 18, 2003·Environmental Science and Pollution Research International·Signe ViggorAin Heinaru
Mar 25, 1985·FEBS Letters·D J HopperM J Causer
Jan 1, 1990·Microbial Ecology·G S SaylerJ M King
Nov 1, 1989·Applied and Environmental Microbiology·J EwersD Freier-Schröder
Apr 1, 1985·Applied and Environmental Microbiology·D J MonticelloW R Finnerty
Nov 11, 2003·Journal of Microbiological Methods·Howard Junca, Dietmar H Pieper
Sep 13, 2018·Journal of Agricultural and Food Chemistry·Daisuke AndoToshiyuki Katagi

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