Regulation of Escherichia coli adenylate cyclase activity during hexose phosphate transport.

Microbiology
V DumayM Crasnier

Abstract

In Escherichia coli, cAMP levels vary with the carbon source used in the culture medium. These levels are dependent on the cellular concentration of phosphorylated EnzymeIIAglc, a component of the glucose-phosphotransferase system, which activates adenylate cyclase (AC). When cells are grown on glucose 6-phosphate (Glc6P), the cAMP level is particularly low. In this study, we investigated the mechanism leading to the low cAMP level when Glc6P is used as the carbon source, i.e. the mechanism preventing the activation of AC by phosphorylated EnzymeIIAglc. Glc6P is transported via the Uhp system which is inducible by extracellular Glc6P. The Uhp system comprises a permease UhpT and three proteins UhpA, UhpB and UhpC which are necessary for uhpT gene transcription. Controlled expression of UhpT in the absence of the regulatory proteins (UhpA, UhpB and UhpC) allowed us to demonstrate that (i) the Uhp regulatory proteins do not prevent the activation of AC by direct interaction with EnzymeIIAglc and (ii) an increase in the amount of UhpT synthesized (corresponding to an increase in the amount of Glc6P transported) correlates with a decrease in the cAMP level. We present data indicating that Glc6P per se or its degradation is unlikely...Continue Reading

References

Jan 1, 1979·Journal of General Microbiology·J G Harman, J L Botsford
Apr 1, 1977·Journal of Bacteriology·C B BruniF Blasi
Oct 1, 1992·Journal of General Microbiology·R J KadnerC M Stephens
Aug 1, 1991·Journal of General Microbiology·F BivilleF Gasser
Sep 1, 1988·Archives of Biochemistry and Biophysics·A Peterkofsky
Aug 1, 1988·Journal of Bacteriology·L A Weston, R J Kadner
Jan 1, 1987·Annales De L'Institut Pasteur. Microbiology·O M Bouvet, P A Grimont
Nov 1, 1969·Journal of Bacteriology·B TylerB Magasanik
Mar 28, 1966·Biochimica Et Biophysica Acta·H H Winkler
Sep 26, 1966·Archives of Biochemistry and Biophysics·B M PogellS Shapiro
May 1, 1967·Journal of Bacteriology·D G Fraenkel, S R Levisohn
Aug 1, 1980·Journal of Bacteriology·B R BochnerB N Ames
Jan 1, 1982·Molecular & General Genetics : MGG·E JosephA Ullmann
Mar 25, 1983·Journal of Molecular Biology·A RoyA Ullmann
Feb 1, 1981·Journal of Bacteriology·S R Maloy, W D Nunn
Aug 1, 1993·Journal of Bacteriology·M D Island, R J Kadner

Citations

Oct 26, 2005·Biotechnology Letters·Robert G UpchurchWeineng Zuo
Aug 1, 1997·Trends in Microbiology·A A Neyfakh
Sep 19, 1997·Trends in Microbiology·R K Poole
May 11, 1999·Drug Discovery Today·J A Heinemann
Aug 29, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·D C Hooper
Aug 29, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·L R Peterson
Feb 5, 2003·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Marilyn C Roberts
Apr 20, 2002·Current Opinion in Infectious Diseases·S Kariuki, C A Hart
Dec 13, 2006·Microbiology and Molecular Biology Reviews : MMBR·Josef DeutscherP W Postma
Apr 30, 2008·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Bijan Zakeri, Gerard D Wright
Sep 15, 2007·BMC Systems Biology·Andreas KremlingErnst Dieter Gilles
Mar 2, 2006·Biological & Pharmaceutical Bulletin·Wakano OgawaTomofusa Tsuchiya
Aug 2, 2007·Biological & Pharmaceutical Bulletin·Hidemasa NakaminamiMasanori Sasatsu
Feb 12, 2014·Current Opinion in Microbiology·Ake Västermark, M H Saier
Mar 1, 1997·Microbiology·M Crasnier-MednanskyM H Saier

Related Concepts

UhpA protein, Bacteria
UhpB protein, Salmonella typhimurium
UhpC protein, bacteria
UhpA protein, E coli
UhpC protein, E coli
Cyclic AMP, (R)-Isomer
Adenylate Cyclase
Bacterial Proteins
Biological Transport
Carrier Proteins

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Pediculosis pubis

Pediculosis pubis is a disease caused by a parasitic insect known as Pthirus pubis, which infests human pubic hair, as well as other areas with hair including eye lashes. Here is the latest research.

Rh Isoimmunization

Rh isoimmunization is a potentially preventable condition that occasionally is associated with significant perinatal morbidity or mortality. Discover the latest research on Rh Isoimmunization here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells. It also follows CRISPR-Cas9 approaches to generating genetic mutants as a means of understanding the effect of genetics on phenotype.

Enzyme Evolution

This feed focuses on molecular models of enzyme evolution and new approaches (such as adaptive laboratory evolution) to metabolic engineering of microorganisms. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Pharmacology of Proteinopathies

This feed focuses on the pharmacology of proteinopathies - diseases in which proteins abnormally aggregate (i.e. Alzheimer’s, Parkinson’s, etc.). Discover the latest research in this field with this feed.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.