PMID: 3768960Nov 7, 1986Paper

Regulation of eukaryotic ribosomal RNA transcription by RNA polymerase modification

Cell
E Bateman, Marvin R Paule

Abstract

Forms of RNA polymerase I prepared from growing or encysted Acanthamoeba are equal in the ability to transcribe poly(dl:dC). Polymerase from cysts, whose rRNA genes are transcriptionally inactive, is unable to utilize the rDNA promoter in vitro, whereas the transcription initiation factor from cysts is fully able to bind the promoter and direct transcription. Footprinting shows that polymerase from cysts is functionally inactive because of its inability to bind to the promoter. The polymerase footprint moves downstream the appropriate number of base pairs upon various nucleotide additions, without affecting the factor footprint. These results support our hypothesis that rRNA synthesis in eukaryotes is regulated by polymerase I modification and not by alterations to additional DNA-binding proteins.

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Citations

Aug 1, 1993·Molecular and Cellular Endocrinology·M GlibeticB H Sells
Jan 8, 1999·Biochimica Et Biophysica Acta·T Orfeo, E Bateman
Oct 19, 1999·Biochimica Et Biophysica Acta·T OrfeoE Bateman
Sep 11, 1991·Nucleic Acids Research·K HisatakeM Muramatsu
Dec 1, 1992·The British Journal of Dermatology·A A VerstraetenJ Schalkwijk
Apr 1, 1991·Molecular Microbiology·J Köck, A W Cornelissen
Jul 1, 1990·The Journal of protozoology·T J ByersV J Stewart
Jan 1, 1997·Annual Review of Microbiology·M G Lee, L H Van der Ploeg
Apr 12, 2000·Proceedings of the National Academy of Sciences of the United States of America·B MoorefieldR H Reeder
Jun 16, 2004·Current Opinion in Genetics & Development·Tom Moss
Jan 1, 1993·Trends in Cardiovascular Medicine·W Xie, L I Rothblum
Apr 1, 2004·Biochimica Et Biophysica Acta·Li ChenErik Bateman
Mar 22, 2005·Biochimica Et Biophysica Acta·Zhihua PengErik Bateman
Sep 23, 1988·Cell·E Bateman, M R Paule
Jun 15, 2000·Nature Cell Biology·M Carmo-FonsecaI Campos
Aug 5, 2005·American Journal of Physiology. Cell Physiology·Gustavo A NaderKaryn A Esser
Dec 17, 2008·The Journal of Biological Chemistry·Masayasu Nomura
Dec 22, 2006·American Journal of Physiology. Cell Physiology·F KawanoY Ohira
Feb 6, 1999·The Journal of Biological Chemistry·H M Alzuherri, R J White
Mar 7, 1997·The Journal of Biological Chemistry·R J Gaudino, C S Pikaard
Sep 6, 1996·The Journal of Biological Chemistry·E M ClarkeD L Riggs
Mar 21, 2003·The Journal of Biological Chemistry·Iwona Hirschler-LaszkiewiczLawrence I Rothblum

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