Regulation of exotoxin A synthesis in Pseudomonas aeruginosa: characterization of toxA-lacZ fusions in wild-type and mutant strains

Molecular Microbiology
M L VasilR W Prince

Abstract

A mobilizable plasmid which carries the promoter for the exotoxin A (ETA) structural gene fused to lacZ was integrated into the chromosome of wild-type and mutant strains of Pseudomonas aeruginosa at the toxA locus by homologous recombination. beta-galactosidase synthesis in the strains (cointegrates) carrying the toxA-lacZ fusions was regulated like ETA synthesis is in P. aeruginosa. Two multicopy plasmids carrying a positive regulatory gene designated toxR were constructed which are identical except with respect to the orientation of toxR to the lacZ promoter on the plasmid. These plasmids were then introduced into P. aeruginosa cointegrate strains. When toxR was using its own promoter, synthesis of beta-galactosidase in the cointegrate strains was increased but the pattern of iron regulation was not altered. In contrast, when the lacZ promoter was directing synthesis of the toxR product in the cointegrate strains, iron regulation of beta-galactosidase and ETA synthesis were abolished.

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Citations

Apr 8, 2010·Expert Reviews in Molecular Medicine·Thomas BjarnsholtMichael Givskov
Mar 16, 2007·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Thomas Bjarnsholt, Michael Givskov
Jul 12, 2013·Future Microbiology·Tim Holm JakobsenNiels Høiby
Jan 24, 2008·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Thomas BjarnsholtMichael Givskov
Sep 14, 2017·International Journal of Molecular Sciences·Tim Holm JakobsenMichael Givskov

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