Abstract
We have evaluated the effects of rmIL-12 on the course of adoptively transferred EAE. When mice were injected with LNC that had been stimulated in vitro with PLP in the presence of rmIL-12, the subsequent course of disease was more severe and prolonged than controls. In vitro stimulation with PLP in the presence of IL-12 was associated with an increase in IFN-gamma and decrease in IL-4-producing cells, indicating a preferential expansion of Th1 effector cells. At peak disease, no notable differences in either the cellular composition or cytokine expression within CNS lesions was seen between groups. However, the frequency of macrophages that stained positively for inducible nitric oxide synthase (iNOS) was increased in animals challenged with rmIL-12 treated LNC. These data suggest that in addition to promoting the preferential expansion of IFN-gamma-producing cells by rmIL-12 treatment in vitro, in vivo effects leading to macrophage activation and iNOS expression may contribute to the severe, protracted course of CNS inflammation in this model. In contrast, treatment of mice with an antibody to murine IL-12 following cell transfer completely prevented paralysis with only 40% of the mice developing mild disease. These data sugg...Continue Reading
References
Sep 1, 1975·Proceedings of the Society for Experimental Biology and Medicine·S Levine, R Sowinski
Jan 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J E MerrillF M Hofman
Feb 1, 1992·Journal of Neuroimmunology·T T DuongG H Strejan
Sep 1, 1989·The Journal of Experimental Medicine·M KobayashiG Trinchieri
Feb 1, 1989·The Journal of Experimental Medicine·I A MacPheeD W Mason
Jun 1, 1994·European Journal of Immunology·O RottE Cash
Nov 1, 1994·The Journal of Experimental Medicine·M K RackeM Röcken
Jan 1, 1995·The Journal of Experimental Medicine·J P LeonardS J Goldman
Mar 10, 1995·Cell·V K KuchrooL H Glimcher
Jan 1, 1993·The Journal of Experimental Medicine·J L BaronC A Janeway
Feb 1, 1994·Journal of Leukocyte Biology·M J Brunda
Mar 1, 1994·Stem Cells·S F WolfJ Sypek
Jun 1, 1993·Journal of Neuroimmunology·M S HarbuzM L Cuzner
Citations
Jun 15, 2007·Modern Rheumatology·Xinfang HuangShunle Chen
Sep 18, 1998·Neuroscience Letters·P BaronM A Cassatella
Feb 15, 2001·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·M BozzaW L Trepicchio
Mar 6, 2004·Hybridoma and Hybridomics·Jiali LiAlan L Epstein
Aug 30, 2001·International Immunology·G T TranS J Hodgkinson
Apr 16, 1998·Transplantation·O IchiguchiM Ogawa
Nov 30, 2006·Transplantation·Nirupama D VermaBruce M Hall
Aug 17, 2004·Journal of Virology·Masanori MatsuiToshitaka Akatsuka
Jun 19, 2002·The Journal of Clinical Investigation·Masha Fridkis-HareliJack L Strominger
May 4, 2006·The Journal of Clinical Investigation·Yi ChenDaniel J Cua
Jan 24, 2014·Clinical & Developmental Immunology·Simone Kennedy BedoyaJoseph Larkin
Jan 25, 2005·Proceedings of the National Academy of Sciences of the United States of America·Joel N H SternJack L Strominger
Apr 20, 2001·Journal of Neuroimmunology·H LiuR R Voskuhl
Jul 26, 2003·Expert Opinion on Biological Therapy·Ruth Cordoba-Rodriguez, David M Frucht
Feb 19, 2008·Pharmacology & Therapeutics·Bhanu P PappuChen Dong
Oct 19, 2004·Neuroscience·N HikawaT Takenaka
Jun 13, 2006·Journal of Neuroimmunology·Nele BerghmansHubertine Heremans
Jul 3, 1998·Journal of Autoimmunity·C C ChanY Zhai
Mar 1, 2003·Nature·Daniel J CuaJonathon D Sedgwick
Feb 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Paresh ThakkerSuzana Marusic
Jul 13, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Paresh ThakkerMina Hassan-Zahraee
Jan 26, 1999·Annals of the New York Academy of Sciences·A BilliauP Matthys
Aug 8, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·S M Liva, R R Voskuhl