Regulation of expression of a sheep metallothionein 1a-sheep growth hormone fusion gene in transgenic mice.

Molecular and Cellular Biology
C M ShanahanK A Ward

Abstract

Transgenic mice containing a sheep metallothionein 1a-sheep growth hormone fusion gene exhibited low, tissue-specific basal levels of transgene mRNA expression, resulting in slightly elevated levels of circulating growth hormone that did not lead to a detectable increase in growth. After zinc stimulation, high levels of transgene mRNA expression were induced in a number of tissues; these levels correlated with increased levels of circulating growth hormone, resulting in growth increases of up to 1.5 times the levels of controls and unstimulated transgenic mice. After removal of the zinc stimulus, transgene expression and circulating growth hormone concentrations returned to basal levels. Additional evidence from the pattern of developmental expression of the transgene suggests that zinc is the main regulator of this promoter in mice. The demonstrated regulation and low basal level of expression of the sheep metallothionein 1a promoter make it a candidate for use in other mouse transgenic studies and for use in transgenic livestock, in which regulation of expression is essential.

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Citations

Apr 8, 2011·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Darlene E BerrymanJohn J Kopchick
Jun 1, 1999·Nutrition Research Reviews·K A Ward
Sep 15, 1991·Experientia·K A Ward, C D Nancarrow
Jul 6, 2000·Journal of Physiology and Biochemistry·M E López-OlivaE Muñoz-Martínez
Apr 1, 1996·Immunology and Cell Biology·A Saberivand, P M Outteridge
Oct 1, 1993·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·E J EisenJ J Kopchick
Oct 6, 2005·Transgenic Research·A D ThomasA M Oberbauer
Mar 23, 2000·Theriogenology·J D Murray

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