Regulation of expression of microvillus membrane proteins by estrogen in baboon fetal ovarian oocytes.

Biology of Reproduction
Nicholas C ZachosGerald J Pepe

Abstract

We previously demonstrated that the number and height of oocyte microvilli were reduced in baboon fetuses deprived of estrogen in utero and restored to normal in animals supplemented with estradiol. Phosphorylated ezrin and Na+/H+ exchange regulatory factor 1 (NHERF, now termed SLC9A3R1) link f-actin bundles to the membrane, whereas alpha-actinin cross-links f-actin to form microvilli. Therefore, we determined whether these proteins were expressed in oocytes of the fetal baboon ovary and whether expression and/or localization were altered between mid and late gestation in association with an increase in estrogen and in late gestation in animals in which estrogen was suppressed (>95%) or restored by treatment with an aromatase inhibitor with or without estradiol. Expression of alpha-actinin was low at mid gestation, increased on the surface of oocytes of primordial follicles in late gestation, and was negligible in the ovaries of estrogen-suppressed fetuses and normal in animals treated with estrogen. Ezrin (total and phosphorylated) and SLC9A3R1 expression was localized to the surface of oocytes at mid and late gestation in estrogen-replete baboons and to the cytoplasm in late gestation after estrogen suppression. These results...Continue Reading

References

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Citations

Oct 31, 2009·The International Journal of Developmental Biology·Eugene D Albrecht, Gerald J Pepe
Dec 7, 2011·Reproduction : the Official Journal of the Society for the Study of Fertility·Purvi V MandeVrinda V Khole
Sep 6, 2011·Reproductive Biomedicine Online·Purvi V MandeVrinda V Khole
Jun 28, 2015·Clinical & Experimental Metastasis·Jiang WangLei Huang

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Methods Mentioned

BETA
light microscopy
electrophoresis
laser capture microdissection

Software Mentioned

MetaMorph Image Analysis system
Image J

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