Regulation of expression of rice thaumatin-like protein: inducibility by elicitor requires promoter W-box elements.

Plant Cell Reports
Kanzaki Hiroyuki, Ryohei Terauchi

Abstract

Rice thaumatin-like protein (Rtlp1) is a high-molecular-weight antimicrobial pathogenesis-related protein that plays a role in plant stress response. This study examines transcriptional regulation of Rtlp1 using wild type and transgenic rice plants carrying a beta-glucuronidase (GUS) reporter gene driven by the Rtlp1 promoter (pRtlp1GUS). The Rtlp1 promoter is induced within 6 h after infection with rice blast fungus (Magnaporthe grisea). The Rtlp1 promoter is also induced by salicylic acid (SA), methyl jasmonate (MeJA), wounding or an elicitor from rice blast fungus. The function of the pRtlp1GUS reporter gene was analyzed by deletion mapping and transient expression assays in cell culture. A 120 bp truncated fusion construct with six W-boxes (5'-TGAC-3') demonstrated a strong dose-dependent elicitor-response. These results suggest that W-box elements are required for the response of the Rtlp1 promoter to fungal elicitors.

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Citations

Oct 19, 2010·Biotechnology Letters·Huiquan ZhengZhiyi Zhang
Sep 4, 2012·International Journal of Molecular Sciences·Marco BettiAntonio J Márquez
Jan 7, 2014·Plant Science : an International Journal of Experimental Plant Biology·Ying GaoKai-Jun Zhao
Jun 22, 2017·Proceedings of the National Academy of Sciences of the United States of America·Surinder SinghJaswinder Singh
Jan 11, 2018·PloS One·Iván BalicReinaldo Campos-Vargas
Sep 12, 2020·Scientific Reports·Saritha Panthapulakkal NarayananMee-Len Chye

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