Regulation of expression of the vanD glycopeptide resistance gene cluster from Enterococcus faecium BM4339

Journal of Bacteriology
B CasadewallP Courvalin

Abstract

A new open reading frame, encoding a putative integrase-like protein, was detected downstream from the six genes of the vanD glycopeptide resistance cluster in Enterococcus faecium BM4339 (B. Casadewall and P. Courvalin, J. Bacteriol. 181:3644-3648, 1999). In this cluster, genes coding for the VanR(D)-VanS(D) two-component regulatory system were cotranscribed from the P(R(D)) promoter, whereas transcription of the vanY(D), vanH(D), vanD, vanX(D), and intD genes was initiated from the P(Y(D)) promoter located between vanS(D) and vanY(D) (the D subscript indicates that the gene is part of the vanD operon). The VanR(D)-VanS(D) regulatory system is likely to activate transcription of the resistance genes from the promoter P(Y(D)). Glycopeptide-susceptible derivatives of BM4339 were obtained by trans complementation of the frameshift mutation in the ddl gene, restoring functional D-alanine:D-alanine ligase activity in this strain. The glycopeptide-susceptible transformant BM4409, producing only D-alanyl-D-alanine-terminating peptidoglycan precursors, did not express the resistance genes encoding the VanY(D) D,D-carboxypeptidase, the VanH(D) dehydrogenase, the VanD ligase, the VanX(D) D,D-dipeptidase, and also the IntD integrase, alt...Continue Reading

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Citations

Aug 29, 2007·Antimicrobial Agents and Chemotherapy·M-C DomingoM G Bergeron
Sep 27, 2006·Antimicrobial Agents and Chemotherapy·Koichi TanimotoYasuyoshi Ike
Dec 25, 2002·Antimicrobial Agents and Chemotherapy·Florence DepardieuPatrice Courvalin
Sep 25, 2004·Antimicrobial Agents and Chemotherapy·Florence DepardieuPatrice Courvalin
Aug 26, 2004·Antimicrobial Agents and Chemotherapy·Isabelle MarchandThierry Lambert
Jan 16, 2007·Clinical Microbiology Reviews·Florence DepardieuPatrice Courvalin
Nov 12, 2002·Journal of Bacteriology·Lorena Abadía PatiñoBruno Perichon
Jun 26, 2007·Molecular Genetics and Genomics : MGG·Tatsuya FukushimaJunichi Sekiguchi
Jul 26, 2002·International Journal of Medical Microbiology : IJMM·Brigitte Berger-Bächi
Feb 13, 2008·FEMS Microbiology Reviews·Eric SauvagePaulette Charlier
Jan 5, 2008·FEMS Microbiology Reviews·Sina JordanThorsten Mascher
Oct 24, 2017·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Mohamed O Ahmed, Keith E Baptiste
Aug 17, 2018·The Journal of Antimicrobial Chemotherapy·Thomas Arn HansenKristian Schønning
Mar 9, 2019·Microbiology Spectrum·Keith E Weaver

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