Regulation of expression within a gene family. The case of the rat gammaB- and gammaD-crystallin promoters.

The Journal of Biological Chemistry
E J KlokN H Lubsen

Abstract

The six closely related and clustered rat gamma-crystallin genes, the gammaA- to gammaF-crystallin genes, are simultaneously activated in the embryonic lens but differentially shut down during postnatal development with the gammaB-crystallin gene, the last one to be active. We show here that developmental silencing of the gammaD-crystallin promoter correlates with delayed demethylation during lens fiber cell differentiation. Methylation silencing of the gammaD-crystallin promoter is a general effect and does not require the methylation of a specific CpG, nor does methylation interfere with factor binding to the proximal activator. In later development, the gammaD-crystallin promoter is also shut down earlier by a repressor that footprints to the -91/-78 region. A factor with identical properties is present in brain. Hence, a ubiquitous factor has been recruited as a developmental regulator by the lens. All gamma-crystallin promoters tested contain upstream silencers, but at least the gammaB-crystallin silencer is distinct from the gammaD-crystallin silencer. The gamma-crystallin promoters were found to share a proximal activator (the gamma-box; around -50), which behaves as a MARE. The gammaB-box is recognized with much lower a...Continue Reading

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Citations

Jul 17, 2012·Cell Stress & Chaperones·Sanne M M HensenNicolette H Lubsen
Jan 17, 2013·Cell Stress & Chaperones·Sanne M M HensenNicolette H Lubsen
Feb 14, 2002·Nucleic Acids Research·Azem CivilNicolette H Lubsen
May 21, 2003·Genes & Development·Claire R WestonRoger J Davis
Jul 8, 2014·Progress in Retinal and Eye Research·Solon ThanosArndt Heiligenhaus
Oct 2, 2007·Progress in Retinal and Eye Research·Ales Cvekl, Melinda K Duncan
Nov 20, 2016·Cellular and Molecular Neurobiology·Yong Wang, Huaijin Guan
Oct 30, 2001·The Journal of Biological Chemistry·Eleonora CarosaJoram Piatigorsky
May 24, 2006·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Tae YamamotoMasayuki Yamamoto
Dec 19, 2001·Experimental Eye Research·L DoerwaldN H Lubsen
Dec 4, 2010·Developmental Biology·Rachel K TittleJeffrey M Gross

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