Regulation of FATTY ACID ELONGATION1 expression in embryonic and vascular tissues of Brassica napus

Plant Molecular Biology
Hélène ChironT J Roscoe

Abstract

The expression of the FATTY ACID ELONGATION1 genes was characterised to provide insight into the regulation of very long chain fatty acid (VLCFA) biosynthesis in Brassica napus embryos. Each of the two rapeseed homoeologous genes (Bn-FAE1.1 and Bn-FAE1.2) encoding isozymes of 3-keto-acylCoA synthase, a subunit of the cytoplasmic acyl-CoA elongase complex that controls the production of elongated fatty acids, are expressed predominantly in developing seeds. The proximal regions of the Bn-FAE1.1 and Bn-FAE1.2 promoters possess strong sequence identity suggesting that transcriptional control of expression is mediated by this region which contains putative cis-elements characteristic of those found in the promoters of genes expressed in embryo and endosperm. Histochemical staining of rapeseed lines expressing Bn-FAE1.1 promoter:reporter gene fusions revealed a strong expression in the embryo cotyledon and axis throughout the maturation phase. Quantitative analyses revealed the region, -331 to -149, exerts a major control on cotyledon specific expression and the level of expression. A second region, -640 to -475, acts positively to enhance expression levels and extends expression of Bn-FAE1.1 into the axis and hypocotyl but also act...Continue Reading

References

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Citations

Nov 22, 2018·Plant & Cell Physiology·Thomas James RoscoeMartine Devic

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Datasets Mentioned

BETA
KF999615.1
AF274750
AF490460.1
AF275254
CDY09507
GR454096
FY432873
KP294338
KP294339

Methods Mentioned

BETA
PCR
protein assays
transgenic
electrophoresis
transfection
genotyping
fluorescence microscopy

Software Mentioned

Primer
DAISY
ClustalW

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