PMID: 7026976Jan 1, 1981Paper

Regulation of ferric iron transport in Escherichia coli K12: isolation of a constitutive mutant

Molecular & General Genetics : MGG
K Hantke

Abstract

The lac genes were inserted with phage Mu(Ap, lac) into the fhuA, fepA, cir and tonB genes which specify components of iron uptake systems. The expression of lac in all these operon fusions was controlled by the availability of iron to the cells, thereby facilitating a quick and simple measurement of the expression of the genes listed above. In an iron rich medium under anaerobic conditions all systems were strongly repressed. fhuA was depressed at higher iron concentration than was fepA or cir, and tonB was repressed only under anaerobic conditions and could be induced by iron limitation. Mutants constitutive for the expression of beta-galactosidase were selected in a fhuA-lac fusion strain. The outer membrane proteins Cir, FhuA, FecA, 76K and 83K were made constitutively in such mutant strains. Therefore, they were termed fur mutants. In these fur mutant strains, the synthesis of a 19K protein was reduced. Furthermore, it was found that transport of ferric enterochelin and ferrichrome was also constitutive in the fur mutant cells, and that ferric citrate uptake could be induced by only 10 microM citrate in the growth medium in contrast to wild-type cells in which at least 100 microM citrate was necessary. The fepA gene was co...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·M J Casadaban, S N Cohen
Nov 30, 1973·Biochimica Et Biophysica Acta·G E Frost, H Rosenberg

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Citations

Jan 1, 1990·Journal of Basic Microbiology·B B Griffiths
Jan 6, 2000·Archives of Biochemistry and Biophysics·D Touati
Jan 1, 1990·Archives of Microbiology·E FischerV Braun
Jan 1, 1985·Molecular & General Genetics : MGG·S SchäfferV Braun
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Nov 4, 2011·Seminars in Immunopathology·James E Cassat, Eric P Skaar
May 24, 2006·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Shelley M PayneNicola M L Davies
May 24, 2006·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·A W Maresso, Olaf Schneewind
May 27, 1994·Gene·E K Hickey, N P Cianciotto
May 15, 2002·Journal of Biotechnology·Joachim JoseFrank Hannemann
Jun 26, 2003·Trends in Microbiology·Jörgen Johansson, Pascale Cossart
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Jul 26, 2002·Molecular Microbiology·Sangpen ChamnongpolEduardo A Groisman
Oct 2, 2002·Molecular Microbiology·Noel BaichooJohn D Helmann
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Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·K Postle, R F Good
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·P C SalinasJ H Crosa

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