Regulation of flagellar bending by cAMP and Ca2+ in hamster sperm

Molecular Reproduction and Development
Fugaku AokiK Kohmoto

Abstract

Hamster sperm were immotile in the medium at free Ca2+ concentrations ([Ca2+]) below 1 x 10(-4) M. The flagellum was acutely bent in the opposite direction to the curve of the hook-shaped heads. This phenomenon seemed to be caused by the decrease in the intracellular cAMP concentration, since the cAMP concentration was low at [Ca2+] below 1 x 10(-4) M and increased abruptly at 1 x 10(-3) M, at which sperm were swimming actively. In addition, sperm became motile due to treatment with 8-bromo-cAMP, a membrane permeable analogue of cAMP, in a medium without Ca2+. These results suggested that extracellular Ca2+ is involved in the regulation of flagellar movement via increasing intracellular cAMP concentration. By the treatment with W-13, a calmodulin inhibitor, sperm also became motile, although cAMP concentration remained at a low level. These results suggested that cAMP is not always required for the flagellar movement when the function of calmodulin is depressed.

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Citations

Feb 20, 2010·Bulletin of Mathematical Biology·Sarah D OlsonLisa J Fauci
Jan 3, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·K AshizawaY Tsuzuki
Mar 26, 2003·Reproduction in Domestic Animals = Zuchthygiene·S S Suarez, H-C Ho
Jul 28, 2011·Journal of Cellular Physiology·Jessica EscoffierChristophe Arnoult
Apr 21, 2016·Reproduction in Domestic Animals = Zuchthygiene·P CastellanosR Mateo
Jun 29, 2006·Molecular Reproduction and Development·Sumio IshijimaAshley I Yudin
Jun 17, 2010·Physiology·Dejian Ren, Jingsheng Xia
May 8, 2018·Journal of Basic and Clinical Physiology and Pharmacology·Teesta V KatteRavindranath H Aladakatti

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