Regulation of flagellar length in Chlamydomonas

Cell Motility and the Cytoskeleton
J TuxhornW L Dentler

Abstract

The length of eukaryotic cilia and flagella depends on the cell cycle-regulated assembly and disassembly of at least 9 doublet and 2 central microtubules, their associated proteins, and the surrounding membrane. In light-synchronized Chlamydomonas cells, flagella assembled to 10-14 microm in length near the beginning of the light period and they disassembled prior to cell division, during the dark period. Flagella on light-synchronized pf18 Chlamydomonas mutants grew to 10-12 microm near the beginning of the light period but shortened by 50% or more by the end of the light period. Flagellar length was cell-cycle regulated: when flagella were amputated at various times during the light period, new flagella regenerated to the lengths of control cells at that time of the light cycle. The later in the cycle pf18 cells were deflagellated, the shorter were the regenerated flagella. Flagellar shortening was not affected, in either pf18 or wild-type (wt) cells, by inhibitors of protein synthesis or of microtubule assembly, so flagellar length cannot depend on protein turnover. Shortening in pf18 was attenuated by Li+, which stimulated flagellar growth in wt cells, by red light, by protein kinase inhibitors, and by the Ca2+ channel bloc...Continue Reading

References

Nov 1, 1979·The Journal of Physiology·H Machemer, A Ogura
Nov 22, 1976·Biochemical and Biophysical Research Communications·B Bhattacharyya, J Wolff
Mar 1, 1991·Journal of Neuroscience Research·B A Hollander, G S Bennett
Jul 1, 1991·Journal of Neurochemistry·G S BennettC DiLullo
Oct 1, 1991·Developmental Biology·C Nocente-McGrathS G Ernst
Jan 1, 1992·Life Sciences·J K HsiaoW Z Potter
Mar 1, 1991·The Biochemical Journal·R ColomboI Dalle Donne
Aug 1, 1991·The American Journal of Physiology·M KatoJ D Vincent
Jul 15, 1990·Biological Psychiatry·D K Welsh, M C Moore-Ede
Jan 1, 1990·The Journal of Cell Biology·P LamoureuxS R Heidemann
Jan 1, 1990·Life Sciences·C C Hale, R S Keller
Jan 1, 1989·Cell Motility and the Cytoskeleton·J S Tash
May 1, 1989·The Journal of Cell Biology·M A Sanders, J L Salisbury
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·B T Livingston, F H Wilt
Jul 17, 1989·Brain Research·H Klemfuss, D F Kripke
Nov 1, 1987·The Journal of Cell Biology·S M Pasquale, U W Goodenough
Mar 1, 1989·Developmental Biology·K R Kao, R P Elinson
Jan 1, 1986·Annual Review of Cell Biology·P A Lefebvre, J L Rosenbaum
Apr 1, 1986·Experimental Cell Research·P A Detmers, J Condeelis
Dec 1, 1980·The Journal of Cell Biology·S E Gitelman, G B Witman
Jan 1, 1981·International Review of Cytology·W L Dentler
May 1, 1981·Acta Pharmacologica Et Toxicologica·P Thams, A Geisler
Mar 1, 1980·The Journal of Cell Biology·D A MeslandU W Goodenough
Jan 1, 1995·Methods in Cell Biology·P A Lefebvre
Oct 1, 1995·The Journal of Cell Biology·G J PazourG B Witman
Jan 1, 1993·Cell Motility and the Cytoskeleton·U W Goodenough
Jul 1, 1993·The Journal of Cell Biology·T SaitoU W Goodenough
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·K G KozminskiJ L Rosenbaum

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Citations

Mar 9, 1999·Molecular Biology of the Cell·M E PorterW Dentler
Aug 13, 2003·Eukaryotic Cell·Martin WieseChristoph G Grünfelder
Oct 8, 2004·Eukaryotic Cell·Nedra F Wilson, Paul A Lefebvre
Feb 10, 2006·Molecular Biology of the Cell·Maja ErdmannMartin Wiese
Jun 19, 2007·Eukaryotic Cell·Goran PerizLaura R Keller
Jun 28, 2011·The Journal of Biological Chemistry·Jianfeng LinDaniela Nicastro
Oct 12, 2004·Annual Review of Cell and Developmental Biology·Wallace F Marshall
Nov 12, 2003·The Journal of Cell Biology·Lai-Wa TamPaul A Lefebvre
Nov 7, 2015·Molecular Biology of the Cell·Richard John Wheeler
Mar 2, 2012·ACS Chemical Biology·Prachee AvasthiWallace F Marshall
Aug 26, 2009·Biochemical and Biophysical Research Communications·Ko MiyoshiMasato Asanuma
Jun 6, 2007·International Journal for Parasitology·Martin Wiese
Jan 29, 2011·Molecular Biology of the Cell·Neeraj SharmaBradley K Yoder
Feb 7, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Qiong WuJunmin Pan
Oct 2, 2013·Planta·Arash Kianianmomeni, Armin Hallmann
Feb 13, 2016·Cellular and Molecular Life Sciences : CMLS·Yinwen LiangJunmin Pan
Nov 24, 2017·Journal of Cellular and Molecular Medicine·Wenlong ZhaoFei Sun
Jun 1, 2001·The Journal of Biological Chemistry·L Song, W L Dentler
Apr 17, 2021·Cellular and Molecular Life Sciences : CMLS·Cenna Doornbos, Ronald Roepman
Sep 11, 2002·Trends in Cell Biology·Wallace Marshall
Aug 2, 2005·Molecular and Biochemical Parasitology·Christine AdhiamboJonathan H LeBowitz
Oct 25, 2013·Biochemistry·Rita Werner-Peterson, Roger D Sloboda
Jul 7, 2009·Experimental Cell Research·Young OuFrans A van der Hoorn

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