Regulation of flagellar motility by temperature-dependent phosphorylation of a 43 kDa axonemal protein in fowl spermatozoa.

Biochemical and Biophysical Research Communications
K AshizawaY Tsuzuki

Abstract

Phosphorylation of fowl sperm proteins was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) after incubating the demembranated spermatozoa with [gamma-32P]ATP at 30 degrees C or 40 degrees C. A marked difference of phosphorylation between 30 degrees C and 40 degrees C was observed in a 43 kDa protein. This protein was slightly phosphorylated at 40 degrees C, but strongly phosphorylated at 30 degrees C in a cAMP-independent manner. The motility of demembranated spermatozoa was negligible at 40 degrees C, but vigorous movement was observed at 30 degrees C. These results showed that phosphorylation of a 43 kDa protein is likely to be a regulatory step in the maintenance of fowl sperm motility.

References

Nov 1, 1991·Journal of Reproduction and Fertility·M F Thomson, G J Wishart
Jan 1, 1990·Archives of Andrology·G C MajumderM Barua
May 1, 1989·Journal of Reproduction and Fertility·K AshizawaK Okauchi
Jan 1, 1989·Archives of Andrology·C B Lindemann, K S Kanous
May 1, 1989·Journal of Reproduction and Fertility·K AshizawaK Okauchi
Jul 1, 1987·Journal of Reproduction and Fertility·G J Wishart, K Ashizawa
Nov 1, 1987·Journal of Cellular Biochemistry·C J Brokaw
Feb 1, 1981·Fertility and Sterility·D F Katz, J W Overstreet
Feb 1, 1982·Biology of Reproduction·M ChulavatnatolD Wititsuwannakul

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Citations

May 7, 1999·Molecular Reproduction and Development·F AokiK Kohmoto
Jan 12, 1999·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·K AshizawaY Tsuzuki
Jun 1, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·K AshizawaY Tsuzuki
Feb 20, 1999·Biology of Reproduction·L R JohnsonG L Gerton

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