Regulation of flagellar motility of fowl spermatozoa: evidence for the involvement of intracellular free Ca2+ and calmodulin

Journal of Reproduction and Fertility
K AshizawaY Tsuzuki


The possible role of intracellular free Ca2+ and calmodulin in the regulation of fowl sperm motility was investigated using an intracellular Ca2+ chelator, 1,2-bis (2-aminophenoxy) ethane-N,N,N',N'-tetraacetic acid, tetraacetoxymethyl ester (BAPTA/AM) and calmodulin antagonists such as N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide hydrochloride (W-7), N-(6-aminohexyl)-1-naphthalenesulfonamide hydrochloride (W-5) and trifluoperazine. Intact fowl spermatozoa maintained vigorous movement in a Ca(2+)-free medium at 30 degrees C. In contrast, the motility of spermatozoa loaded with BAPTA/AM was negligible at 30 degrees C, but could be instantly restored by the addition of 2 mmol CaCl2 l-1. At this time, the intracellular free Ca2+ concentrations increased from 0 to about 100 nmol l-1, as measured by the fluorescent Ca2+ indicator fura-2. At 40 degrees C, neither control nor BAPTA/AM-treated spermatozoa were motile, but the motility of both kinds of spermatozoa was restored by the subsequent addition of 2 mmol CaCl2 l-1. Even in the presence of 2 mmol CaCl2 l-1, the addition of W-7 and trifluoperazine inhibited the motility of intact spermatozoa at 30 degrees C and 40 degrees C, and induced a concomitant decrease in the rate of ...Continue Reading


Dec 28, 1999·Animal Reproduction Science·G J Wishart, Y I Wilson
Jan 12, 1999·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·K AshizawaY Tsuzuki
Aug 31, 2006·BMC Genomics·Jae Yong HanHeebal Kim
Dec 8, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·K AshizawaY Tsuzuki
Jan 3, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·K AshizawaY Tsuzuki
Jul 11, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·M LemoineE Blesbois
Jan 29, 2016·Parasites & Vectors·Erin M McCammickAaron G Maule
Mar 19, 2016·The FEBS Journal·Thi Mong Diep NguyenElisabeth Blesbois
May 10, 2005·Biology of Reproduction·George G Ignotz, Susan S Suarez
Jan 28, 1998·Molecular Reproduction and Development·K AshizawaY Tsuzuki
May 7, 1999·Molecular Reproduction and Development·F AokiK Kohmoto
Jul 20, 2004·Cell Motility and the Cytoskeleton·Mamoru NomuraMasaaki Morisawa

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